Anti-phospho-c-Raf (pSer³³⁸/pTyr³⁴⁰) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

c-Raf or Raf-1 belongs to the Raf family of kinases that have regulatory functions in cell growth, differentiation, growth factor receptor stimulation via the MAPK signalling. The other members of the family are A-Raf and B-Raf. All the isoforms have unique as well as overlapping regulatory functions. c-Raf kinase is the first identified downstream effector kinase of Ras, and is the activator of MEK which in turn activates ERK. Phosphorylation of c-Raf at Ser³³⁸and Tyr³⁴⁰regulates its biological function.
Anti-phospho-c-Raf [pSer³³⁸/pTyr³⁴⁰] detects human c-Raf [pSer³³⁸/pTyr³⁴⁰]hosphorylated at [pSer³³⁸/pTyr³⁴⁰]er338 and [pSer³³⁸/pTyr³⁴⁰]yr340 (74 kDa). Mouse and [pSer³³⁸/pTyr³⁴⁰]at c-Raf (100% homology) are [pSer³³⁸/pTyr³⁴⁰]xpected [pSer³³⁸/pTyr³⁴⁰]o cross react.

免疫原

synthetic phosphopeptide derived from the region of human c-Raf that contains serine 338 and tyrosine 340.

应用

The antibody may be used to detect phospho-c-Raf (Ser³³⁸/Tyr³⁴⁰) by immunoblotting at working dilution of 1:1000 in cell lysates from HEK293 cells transfected with c-Raf. Detection was also possible in spermatozoa of mice at 1:1000 dilution.

外形

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD06411878
NACRES	NA.44

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous glycerol solution
species reactivity	mouse, rat, human
technique(s)	western blot: 1:1,000 using cell lysates from Hek293 cells transfected with c-Raf, or co-transfected with c-Raf, Ras[V12] and Rac1.
UniProt登记号	P04049
运输	wet ice
储存温度	?20℃
Gene Information	human ... RAF1(5894) mouse ... Zhx2(387609)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)