半岛bd体育手机客户端 说明
一般描述
Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; c-Kit (Stem cell factor receptor, SCF R; CD117) being the most critical. The signalling triggered by c-Kit results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. The secondary pathways activated by c-Kit include Akt, mTOR, MAPK, Sos-Grb2 and c-Jun. Phosphorylation of tyrosine 730 is required for the binding of PLCγ leading to activation of various signalling pathways In addition to development of mast cells, c-Kit also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF R is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
The antibody detects human c-Kit pTyr730. Mouse, rat and cow (100% homologous), dog (92% homologous), chicken (85%) are expected to react.
免疫原
synthetic phosphopeptide derived from the region of human c-Kit that contains tyrosine 730.
应用
Anti-phospho-c-Kit (SCFR) pTyr730antibody may be used for immunoblotting at a working concentration of 0.1-1.0 μg/ml using M07e cells.
外形
Solution in Dulbecco′s phosphate buffered saline (without Mg2+and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD06411871 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| species reactivity | rat, human, mouse |
| technique(s) | western blot: 0.1-1.0 μg/mL using M07e cells |
| UniProt登记号 | P10721 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... KIT(3815) mouse ... Kit(16590) rat ... Kit(64030) |
Sigma-Aldrich
m.cnreagent.com
