半岛bd体育手机客户端 说明
一般描述
Notch signaling is a well conserved among the metazoans and is a regulator of growth, differentiation, and patterning processes. One of the ligands of Notch family receptors is Jagged. Mammalian Jagged family comprises of Jagged-1 and Jagged-2. Jagged proteins are expressed during the critical stages of embryonic and postnatal development. The Notch-Jagged signaling regulates the proliferation of endothelial cells, apoptosis, differentiation of hematopoietic progenitors, skeletal development and formation and differentiation of blood vessels. Notch-jagged pathway components play important role in the pathology of diseases like Alagille syndrome, CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy) and osteosarcoma. Anti-Jagged 1 antibody specifically recognizes rat Jagged-1.
免疫原
recombinant rat Jagged 1 extracellular domain, expressed in NSO cells.
应用
Anti-Jagged 1 antibody may be used for ELISA at a working antibody concentration of 0.5-1.0 μg/ml. For immunoblotting, antibody concentration of 0.1-0.2 μg/ml is recommended. For flow cytometry, a concentration of 20 μg/ml (10 μL/105cells to 106cells) may be used. The antibody is suitable for neutralization assays (ND50 is ~2-10 μg/ml).
目标描述
Jagged-1 is a Notch ligand. The Notch signaling system regulates proliferation, differentiation, and apoptosis in many tissues, including hematopoietic tissue. Notch is a family of transmembrane receptors activated by ligands (Jagged-1, Jagged-2, and Delta-1) that are expressed on adjacent cells. Hematopoietic stem cells and early progenitors express Notch-1 and Notch-2, whereas primary stromal cultures have been shown to express Jagged-1, indicating that Jagged-1 may play a role in nurturing progenitor cells within the hematopoietic microenvironment. Cells engineered to express high levels of Jagged-1 caused a four-fold increase in formation of hematopoietic progenitor cell colonies. However, without the presence of other cytokines that stimulate proliferation and maturation, such as IL-3, IL-6, SCF and G-CSF, Jagged-1 actually decreased myeloid colony formation of CD34+cells, suggesting that Jagged-1 modulates cytokine signals and helps preserve CD34+cells in an immature state.
外形
lyophilized from a 0.2 μm filtered solution of phosphate buffered saline (PBS), pH 7.4, and 5% trehalose
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02096779 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | goat |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | lyophilized powder |
| species reactivity | rat |
| technique(s) | capture ELISA: 0.2-0.8 μg/mL flow cytometry: 2.5 μg using 106rat cortical stem cells western blot: 0.1 μg/mL |
| UniProt登记号 | Q63722 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... JAG1(182) rat ... Jag1(29146) |
安全信息
| 危险声明 | H412 |
| 预防措施声明 | P273 - P501 |
| 危险分类 | Aquatic Chronic 3 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
