Anti-DFF45, C-Terminal antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

DFF exists as an inactive cytoplasmic protein until activated by apoptotic signals. DFF40, the nuclease responsible for DNA fragmentation during apoptosis, exists as a heterodimeric complex with DFF45.

免疫原

synthetic peptide corresponding to amino acids 313-331 of the C-terminal region of human DFF45/ICAD (DNA Fragmentation Factor 45/ Inhibitor of Caspase-3 Activated DNase I).

应用

Anti-DFF45 antibody produced in rabbit is suitable for western blot analysis at a working concentration of 0.25μg/mL using human HeLa cell extract.

生化/生理作用

DFF45 functions as both a chaperone, mediating the correct folding of DFF40, and an inhibitor of DFF40. DFF45 is cleaved by caspase-3 at two sites in response to apoptotic signals. This releases DFF40, the active nuclease. DFF40 seems to oligomerize, forming a large, functional complex which breaks down DNA by introducing double strand breaks. Also, DFF40 appears to interact directly with histone H1 that may stimulate its activity.

外形

Solution in phosphate buffered saline containing 0.02% sodium azide

基本信息

MDL编号	MFCD02096209
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	IgG fraction of antiserum
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 45 kDa (non-apoptotic cells)
species reactivity	human
technique(s)	western blot: 0.25 μg/mL using human HeLa cell extract
UniProt登记号	O00273
运输	dry ice
储存温度	?20℃
Gene Information	human ... DFFA(1676)