Anti-CD137 antibody produced in goat

半岛bd体育手机客户端 说明

一般描述

CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.

免疫原

mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu²⁴Gln¹⁸⁶

应用

Anti-CD137 antibody produced in goat is suitable for the following applications:

• Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
• ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
• Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
• Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
• Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.

生化/生理作用

CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

重悬

Reconstitute at 0.2 mg/mL in sterile PBS

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD01094424
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	goat
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	lyophilized powder
species reactivity	human
technique(s)	capture ELISA: 0.2-0.8 μg/mL flow cytometry: 2.5 μg using 106human T cells treated with PHA immunocytochemistry: 5-15 μg/mL western blot: 0.1 μg/mL
杂质	<0.10 EU/μg endotoxin
UniProt登记号	Q07011
储存温度	?20℃
Gene Information	human ... TNFRSF9(3604)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable