Aminopeptidase I fromStreptomyces griseus

半岛bd体育手机客户端 说明

一般描述

Aminopeptidase I fromStreptomyces griseusis a thermostable enzyme with Glu131 and Tyr246 as key active site residues.

应用

Aminopeptidase I fromStreptomyces griseushas been used:

• to test the biochar exposure effect on the enzyme activity
• in circular dichroism (CD) spectroscopy studies
• as a positive control in p-nitroanilide degradation assay

包装

0.1 mg in serum bottle
Package size based on protein content.

生化/生理作用

Aminopeptidase I may also be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. In the first stage, the endoprotease cleaves a peptide, such as Z-Y-X-Leu-p-nitroanilide, with the X, Y, and Z residues being chosen according to the specificity of the endoprotease.
Aminopeptidase I fromS. griseushas a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn²⁺binding sites. Aminopeptidase I fromS. griseusis inhibited by 1,10-phenanthroline and is activated six-fold by Ca²⁺, which also stabilizes it against heat inactivation. This monomeric zinc metalloprotein has an isoelectric point (pI) of 5.4.

单位定义

One unit will hydrolyze 1.0 μmole of L -leucine-p-nitroanilide to L -leucine and p-nitroaniline per min at pH 8.0, 25 ℃ and 3.0 mM substrate concentration.

外形

Contains calcium acetate

制备说明

Reconstitute in 20 mM tricine, pH 8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use.

其他说明

Endopeptidase contaminant: Not more than: 0.01 U/mg protein (as μmole tyrosine equivalent per min released from casein.)

基本信息

酶学委员会(EC)编号	3.4.11.22 ( BRENDA | IUBMB )
EC 号	232-874-6
MDL编号	MFCD00166325
NACRES	NA.54

半岛bd体育手机客户端 性质

形式	lyophilized powder
质量水平	200
specific activity	≥200 units/mg protein
分子量	21 kDa by gel filtration 33 kDa by SDS-PAGE
组成	Protein, 40-60% Lowry
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H319 - H334 - H335
预防措施声明	P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官	Respiratory system
储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)