Alkaline Phosphatase Blue Membrane Substrate Solution

半岛bd体育手机客户端 说明

一般描述

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.

应用

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:

• to analyse direct alkaline phosphatase (AP) activity
• to perform alkaline phosphatase assays with colorimetric system
• to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Prior to reaction with alkaline phosphatase, the BCIP ^?reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

外形

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP^?) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

法律信息

BCIP is a registered trademark of sigma-aldrich Co. LLC

基本信息

eCl@ss	42010105
NACRES	NA.83

半岛bd体育手机客户端 性质

质量水平	200
形式	solution
用途	sufficient for 400 mL working substrate
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable