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UKKi011-A

品牌
Sigma-Aldrich
货号
66540010
规格纯度
Human iPS Cell Line
价格
11450.3 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
UKKi011-A
EBiSC iPSC Line
iPSC
Human iPSC
iPS Cell
Induced Pluripotent Stem Cell Line
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

细胞系来源

Depositor
Klinikum der Universit?t zu K?ln

细胞系描述

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Geneva, Switzerland
Tissue collection date: March 6, 2013
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
L-Myc
POU5F1
sh-p53
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression:Alkaline Phosphatase(+)SSEA-3(+)TRA 1-60(+)SSEA-4(+)POU5F1 (OCT-4)(+)TRA 1-80(+)NANOG(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:B-TUBULIN(-)HES5(+)NEUROD1(+)PAX6(-)PDGF(+)Sox1(-)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4(+)FOXA2(-)GATA6 (+)GSC(-)PITX1(+)SOX17 (+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:BMP4(+)DCN(+)HAND1(+)PDGF(+)PECAM(+)VIMENTIN(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping

Karyotyping
Passage number: 35
Cell line karyotype: 46,XY
Karyotyping method: Molecular karyotyping using OmniExpress Exome Chip
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

联系

  • Additional Cell Line Info
  • EBiSC Access Use Agreement (EAUA)
  • Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

传代培养常规

Medium: Essential E8?
Passage method: EDTA
Matrix: Vitronectin
CO2concentration: 5%
O2concentration: 21%
Temperature: 37℃

法律信息

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft

半岛bd体育手机客户端 性质

质量水平 100
生物来源 human dermis (fibroblast)
重编程方法 episomal
描述 age (N/A)
manufacturer/tradename EBiSC?
性别 male
生长模式 adherent (pluripotent)
technique(s) cell culture | stem cell: suitable
相关疾病 none
运输 dry ice
储存温度 ?196℃

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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