半岛bd体育手机客户端 说明

一般描述

SYBR^?Green Extract-N-Amp^?Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix^?. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl₂, is specially formulated to amplify target sequences in the cellular lysate.

特点和优势

• Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
• No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
• Specially formulated PCR ReadyMix for use with extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• No loading buffers or tracking dyes required for gel analysis
• SYBR^?Green I dye acts as a nonspecific reporter for real-time PCR
• SYBR^?Green I dye is inexpensive, easy to use, and highly sensitive
• Compatible with high-throughput requirements for genetic analysis of plants
• No long enzymatic digestions
• Extract stable at 4℃ for at least 6 months

其他说明

该实验方案采用20 μl规格的PCR反应。

法律信息

Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

基本信息

NACRES

NA.55

半岛bd体育手机客户端 性质

质量水平	200
用途	sufficient for 100 preparations sufficient for 100 reactions
特点	dNTPs included hotstart
technique(s)	qPCR: suitable
detection method	SYBR?Green
运输	wet ice
储存温度	?20℃

安全信息

象形图
警示用语：	Warning
危险声明	H315 - H317 - H319 - H411
预防措施声明	P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
危险分类	Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable