Immunoprecipitation Vector Kit

半岛bd体育手机客户端 说明

一般描述

The Immunoprecipitation (IP) Vector Kit provides a unique method to validate protein-protein interactions in a convenient 96-well format. This system enables potential binding partners for the protein of interest to be tagged with ”FLAG and c-Myc epitopes, followed by capture and detection using the ANTI-FLAG M2 High Sensitivity Capture Plate.

The kit provides expression vectors ”pFLAG-CMV”-2 and pc-Myc-CMV-2 designed for the cloning and expression of protein interaction candidates as N-terminal FLAG and N-terminal c-Myc fusions. Control plasmids pFLAG-CMV-2-p53, pC-Myc-CMV-2-Large T antigen and pC-Myc-CMV-2-BAP supplied are for expression of positive and negative binding partners in the IP analysis. The kit also includes the MF-2 and MR-2 verification primers.

Vector Maps and Sequences

特点和优势

• Fast and convenient 96 well plate format
• Cleaner analysis due to strongly bound antibody
• Rapid, sensitive, quantitative results

组分

The IP Vector Kit contains:

• pFLAG-CMV-2 Expression Vector (10 μg)
• pc-Myc-CMV-2 Expression Vector (10 μg)
• pFLAG-CMV-2-p53 control plasmid (20 μg)
• pc-Myc-CMV-2-Large T antigen control plasmid (10 μg)
• pc-Myc-CMV-2-BAP control plasmid (10 μg)
• Verification Primer-MF (2 nmol)
• Verification Primer-MR (2 nmol)

基本信息

NACRES

NA.85

半岛bd体育手机客户端 性质

质量水平	200
等级	for molecular biology
形式	buffered aqueous solution
运输	dry ice
储存温度	?20℃

组份列表

组份不可单独销售

货号	组份
	pc-Myc-CMV-2 10 μg
	pc-Myc-CMV-2-BAP 10 μg
	pc-Myc-CMV-2-Large T antigen 10 μg
	pFLAG-CMV?-2 10 μg
	pFLAG-CMV?-2-p53 20 μg
	Verification Primer-MF 2 nmol
	Verification Primer-MR 2 nmol

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable