AFRC 84C6

半岛bd体育手机客户端 说明

细胞系来源

Horse sub-mandibular lymph node x AFRC 17D1

细胞系描述

The heterohybridoma AFRC 84C6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 84C6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

免疫原

Influenza A equine 2 virus

应用

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

培养基

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37℃

其他说明

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半岛bd体育手机客户端 性质

生物来源	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse sub-mandibular lymph node
骨髓瘤	AFRC 17D1
反应性	Anti-Haemagglutinin, influenza
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃