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位置: 首页> 品牌> Sigma-Aldrich> 羟乙基哌嗪乙硫磺酸

HEPES

4-(2-羟乙基)哌嗪-1-乙磺酸

半岛bd体育手机客户端 名称:
7365-45-9
HEPES
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
羟乙基哌嗪乙硫磺酸
4-(2-羟乙基)哌嗪-1-乙磺酸
N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

HEPES has been described as one of the best all-purpose buffers available for biological research.[7] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[1]

应用

HEPES has been used:

  • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo?
  • As a component of Hanks′ solution used in the degradation testing of Iron?
  • As a component of HEPES medium in the transfection of cell culture with plasmids?
  • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells?
  • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies?

基本信息

经验(实验)分子式 C8H18N2O4S
分子量 238.30
Beilstein 883043
MDL编号 MFCD00006158

半岛bd体育手机客户端 性质

质量水平 200
生物来源 synthetic
等级 reagent grade
测定 ≥99% (titration)
形式 crystalline
有效pH范围 6.8 - 8.2
pKa (25 ℃) 7.5
SMILES string OCCN1CCN(CC1)CCS(O)(=O)=O
InChI 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key JKMHFZQWWAIEOD-UHFFFAOYSA-N

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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