半岛bd体育手机客户端 说明
一般描述
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
细胞系来源
Depositor
University of Oxford
细胞系描述
Derivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2010
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes -
PluriTest: Pluripotency score: 27.23
Novelty score: 1.42
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46 XY
Karyotyping method: Molecular karyotyping by SNP array
联系
传代培养常规
Surface coating: Matrigel?/Geltrex?
Passage method: Enzyme-free cell dissociation / EDTA
O2concentration: 21%
CO2concentration: 5%
Culture medium: mTeSR?1
Passage Number banked (pre-EBiSC): 5
No. Vials banked (pre-EBiSC): 3
法律信息
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.
半岛bd体育手机客户端 性质
| 生物来源 | human dermis (fibroblast) |
| 重编程方法 | retrovirus |
| 描述 | age (55-59) |
| manufacturer/tradename | EBiSC? |
| 性别 | male |
| 生长模式 | adherent (pluripotent) |
| technique(s) | cell culture | stem cell: suitable |
| 相关疾病 | Parkinson′s disease |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
