T47D/TR-1

半岛bd体育手机客户端 说明

一般描述

T47D/TR-1 cells are ER alpha positive and express progesterone receptor, although at reduced level compared to parental T7D/S2 cells. T47D/TR-1 cells are growth inhibited by fulvestrant. Oestrogen receptor.

细胞系描述

The T47D/TR-1 is a breast cancer cell line resistant to tamoxifen. The T47D/TR-1 cell line has been established by long term treatment of T47D/S2 cells with 1 μM tamoxifen. Clonal selection was performed in medium without tamoxifen. After growth in presence of 1 μM tamoxifen for 10 months, the growth rate was similar to the parental cells.

培养基

RPMI 1640 w/o Phenol Red (R7509) + 2% FBS (F2442) + 2.5 mM Ala-Gln (G8541) + 8μg/ml Insulin (I9278)

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10, 000 cells/cm? using 0.05% trypsin/EDTA; 5% CO₂; 37℃. Recommend media changes every 2-3 days.

其他说明

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

引用

If use of this culture results in a scientific publication, it should be cited in the publication as: T47D/TR-1 (ECACC 16022513)

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

半岛bd体育手机客户端 性质

生物来源	human breast
生长模式	Adherent
形态学	polygonal epithelia
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃