LIM2550

半岛bd体育手机客户端 说明

细胞系描述

LIM2550 is derived from an adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0169.

培养基

RPMI1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM.

传代培养常规

Optimal split ratio 1:4-1:8 (seeding density 1-2 x 10⁴cells/cm²). Cells incubated at 37℃ with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37℃ for 5 min. LIM2550 requires the growth medium to be changed 3 times each week. Feed and passage the cells as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).

其他说明

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

半岛bd体育手机客户端 性质

生物来源	human colon
描述	Human columnar cell adenocarcinoma of the colon
生长模式	Adherent
形态学	Epithelial; cells grow as floating and loose aggregates with some adherent cells.
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃