半岛bd体育手机客户端 说明
细胞系描述
Human adenocarcinoma of the caecum of a male patient. Cell Bank Australia reference number: CBA0165.
培养基
RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM
传代培养常规
Optimal split ratio 1:4-1:6 (seeding density 1.6 x 104cells/cm2). Cells incubated at 37℃ with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37℃ for 5 min. LIM2405 may take 2-3 days to seed after thawing or trypsinisation.
其他说明
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半岛bd体育手机客户端 性质
| 生物来源 | human colon |
| 生长模式 | adherent |
| 形态学 | epithelial |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
Sigma-Aldrich
m.cnreagent.com
