Raji CD19 Antigen Panel Cell Lines

半岛bd体育手机客户端 说明

一般描述

This product is a panel of three (3) genetically modified cell lines involving the CD19 gene. The panel consists of a CD19 knockout cell line (CD19^-/-) as well as a CD19^lowand a CD19^highexpression cell line. The CD19^-/-cell line was generated using CompoZr^?Zinc Finger Nuclease technology, and the two CD19 expression cell lines were generated using MISSION^?lentivirus. The CD19^lowand CD19^highcell lines express CD19 at low and high levels relative to the CD19^-/-cell line (CD19^high> CD19^low> CD19^-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type Raji cells demonstrate CD19^mediumexpression as measured via FACS.
The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 85011429.

细胞系描述

Established in 1963 from Burkitt′s lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. Raji is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black

特点和优势

The Raji CD19 antigen panel cell lines feature CD19 expressed at varying levels (KO, low, and high). These cell lines enable researchers to screen and evaluate the binding and potency of CD19-targeted immunotherapies in a biologically-relevant cellular research model.

组分

ATG001A-1VL: Raji CD19 Knockout
ATG001B-1VL: Raji CD19 Low
ATG001C-1VL: Raji CD19 High

质量

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.

法律信息

MISSION Use Licenses Agreement

ZFN Use Licenses Agreement
CompoZr is a registered trademark of sigma-aldrich Co. LLC
MISSION is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

生物来源	human lymph (lymphoblast)
生长模式	suspension
形态学	lymphoblast
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	6.2 - Infectious substances
WGK	WGK 2