A549 HLA Panel Cell Lines

半岛bd体育手机客户端 说明

一般描述

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 86012804.
This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in an A549 lung carcinoma parental cell line background. The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines.
CompoZr^?Zinc Finger Nuclease (ZFN) technology was used to generate the β2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS).
MISSION^?lentivirus was used to generate the ten (10) monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a β2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis.

细胞系描述

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

特点和优势

Unlike wild type A549 cells, which express multiple HLA subtypes, the A549 HLA panel cell lines express individual MHC Class I HLA subtypes. These monoallelic HLA cell lines can be used to study peptide binding and presentation in the context of individual HLA subtypes. The panel can also be used to develop cell-based assays to evaluate candidate cancer immunotherapies and identify tumor-specific neoantigens.

组分

HLA003A-1VL: A549 B2M KO Cells
HLA003B-1VL: A549 HLA-A*02:01 Cells
HLA003C-1VL: A549 HLA-A*01:01 Cells
HLA003D-1VL: A549 HLA-A*03:01 Cells
HLA003E-1VL: A549 HLA-A*11:01 Cells
HLA003F-1VL: A549 HLA-A*24:02 Cells
HLA003G-1VL: A549 HLA-B*15:10 Cells
HLA003H-1VL: A549 HLA-B*07:02 Cells
HLA003I-1VL: A549 HLA-B*08:01 Cells
HLA003J-1VL: A549 HLA-B*35:01 Cells
HLA003K-1VL: A549 HLA-B*40:01 Cells

质量

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.

法律信息

MISSION Use Licenses Agreement

ZFN Use Licenses Agreement
CompoZr is a registered trademark of sigma-aldrich Co. LLC
MISSION is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

生物来源	human lung (Carcinoma)
生长模式	Adherent
形态学	Epithelial
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2