A549 EGFR Antigen Panel Cell Lines

半岛bd体育手机客户端 说明

一般描述

This product is a panel of three (3) genetically modified cell lines involving the EGFR gene. The panel consists of a EGFR knockout cell line (EGFR^-/-) as well as a EGFR^lowand a EGFR^highexpression cell line. The EGFR^-/-cell line was generated using CompoZr^?Zinc Finger Nuclease technology, and the two EGFR expression cell lines were generated using MISSION^?lentivirus. The EGFR^lowand EGFR^highcell lines express EGFR at low and high levels relative to the EGFR^-/-cell line (EGFR^high> EGFR^low> EGFR^-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type A549 cells demonstrate EGFR^mediumexpression as measured via FACS.
The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 86012804.

细胞系描述

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

特点和优势

The A549 EGFR antigen panel cell lines feature EGFR expressed at varying levels (KO, low, and high). These cell lines enable researchers to screen and evaluate the efficacy and potency of EGFR-targeted immunotherapies in a biologically-relevant cellular research model.

组分

ATG002A-1VL: A549 EGFR Knockout
ATG002B-1VL: A549 EGFR Low
ATG002C-1VL: A549 EGFR High

质量

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.

法律信息

MISSION Use Licenses Agreement

ZFN Use Licenses Agreement
Combigel is a trademark of sigma-aldrich Co. LLC
MISSION is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

生物来源	human lung (carcinoma)
生长模式	adherent
形态学	epithelial
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2