半岛bd体育手机客户端 说明
一般描述
Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
包装
1 kit in Glass bottle
特点和优势
Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)
组分
WST-1, 1-Methoxy PMS, and a user protocol.
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
半岛bd体育手机客户端 规格
Assay Time: 0.5 - 4 h
原理
The Calbiochem
制备说明
? WST-1 Labeling Mixture:Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.
其他说明
Ishiyama, M., et al. 1995.In Vitro Toxicology8,187.
Liu, S.O., et al 1995.Nat. Med.1,267.
Ishiyama, M., et al. 1993.Chem. Pharm. Bull.41,1118.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
质量水平 | 100 |
用途 | sufficient for 500 tests |
包装 | pkg of 1 96-well plate(s) |
manufacturer/tradename | Calbiochem? |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles protect from light |
input | sample type cultured cells |
试验参数 | sample: 1000 - 50,000 cells |
detection method | colorimetric |
运输 | wet ice |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich