Rapid Cell Proliferation Kit

半岛bd体育手机客户端 说明

一般描述

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

包装

1 kit in Glass bottle

特点和优势

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

组分

WST-1, 1-Methoxy PMS, and a user protocol.

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

半岛bd体育手机客户端 规格

Assay Time: 0.5 - 4 h

原理

The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

制备说明

? WST-1 Labeling Mixture:Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.

其他说明

Ishiyama, M., et al. 1995.In Vitro Toxicology8,187.
Liu, S.O., et al 1995.Nat. Med.1,267.
Ishiyama, M., et al. 1993.Chem. Pharm. Bull.41,1118.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
用途	sufficient for 500 tests
包装	pkg of 1 96-well plate(s)
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles protect from light
input	sample type cultured cells
试验参数	sample: 1000 - 50,000 cells
detection method	colorimetric
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码

10 - Combustible liquids