Polyphosphoinositide-Binding Peptide, PBP10-Calbiochem

半岛bd体育手机客户端 说明

一般描述

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 ?M). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelet without significantly affecting intracellular Ca²⁺influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP₂) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs.
A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 ?M). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelets without significantly affecting intracellular Ca²⁺influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP₂) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Excitation max.: ~565 nm; emission max.: ~590 nm.

包装

1 mg in Glass bottle
Packaged under inert gas

生化/生理作用

Product does not compete with ATP.
Primary Target
Specifically binds to polyphosphoinositide (PPI)
Reversible: yes
Cell permeable: yes

警告

Toxicity: Standard Handling (A)

序列

Rho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH

外形

Supplied as a trifluoroacetate salt.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他说明

Bucki, R., et al. 2001.Biochemistry40,15752
Cunningham, C.C., et al. 2001.J. Biol. Chem.276,43390.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C84H127N24O15
分子量	1713.06

半岛bd体育手机客户端 性质

质量水平	100
测定	≥95% (HPLC)
形式	lyophilized solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze desiccated (hygroscopic) protect from light
颜色	pink-red
溶解性	DMSO: 2 mg/mL
荧光	λex565 nm λem590 nm
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable