MMP/TACE Substrate, FS-6-Calbiochem

半岛bd体育手机客户端 说明

一般描述

The widely used MMP substrate FS-1 (Cat. No. 03-32-5032), modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and sensitivity. Exhibits a 2- to 9-fold higher specificity constant (k_cat/K_m) towards MMP-1, -8, -13, -14 (K_m(μM)= 27.5, 5.2, and 7.9, respectively) when compared with FS-1 and ~ 2-fold higher k_cat/K_m(778, 400 M^-1s^-1) towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10. Excitation max: 324 nm; Emission max: 400 nm.
The widely used MMP substrate FS-1 (Cat. No. 03-32-5032) is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities. Exhibits 2- to 9-fold higher specificity constant (k_cat/K_m) towards MMP-1, -8, -13, -14 when compared with FS-1 and ~2-fold higher k_cat/K_mtowards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10.

包装

1 mg in Glass bottle
Packaged under inert gas

生化/生理作用

Product does not compete with ATP.
Reversible: no
Primary Target
Broad-spectrum MMP and TACE substrate
Cell permeable: no

警告

Toxicity: Standard Handling (A)

序列

Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH?

外形

Supplied as a trifluoroacetate salt.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他说明

Neumann, U., et al. 2004.Anal. Biochem.328,166.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C55H80N16O16
分子量	1221.32

半岛bd体育手机客户端 性质

质量水平	100
测定	≥97% (HPLC)
形式	lyophilized
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze desiccated (hygroscopic) protect from light
溶解性	water: 20 mg/mL
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable