半岛bd体育手机客户端 说明
一般描述
A cell-permeable peptide analog of Hexokinase II VDAC binding domain (HXK2VBD) peptide (Cat. No. 376815). The internalization sequence of the Antennapedia homeoprotein is fused to the N-terminal methionine of the HXK2VBD peptide. Has been shown to cause the complete release of hexokinase II (HXK2) from the mitochondria and subsequent translocation to the cytosol in HeLa cells (100% release at 100 ?M). Does not induce Bax translocation or cytochrome c release. Also shown to markedly sensitize cells to Bax-induced cytochrome c release. Also increases the sensitivity of cells to apoptosis in combination with the Bax-dependent apoptosis inducer, Indomethicin (Cat. No. 405268).
A cell-permeable peptide analog of Hexokinase II VDAC binding domain peptide (Cat. No. 376815). The internalization domain of the Antennapedia homeoprotein is fused to the methionine amino terminal. Shown to completely detach and translocate HXK2 from mitochondria to the cytosol in HeLa cells at 100 ?M. Does not induce Bax translocation or cytochromecrelease when used alone. However, it markedly sensitizes cells to cytochromecrelease and to the induction of apoptosis when used in combination with a Bax-dependent apoptosis inducer, Indomethacin (Cat. No. 405268).
包装
1 mg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Detach and translocate HXK2 from mitochondria to the cytosol
警告
Toxicity: Standard Handling (A)
序列
H-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Met-Ile-Ala-Ser-His-Leu-Leu-Ala-Tyr-Phe-Phe-Thr-Glu-Leu-Asn-NH?
其他说明
Pastorino, J.G., et al. 2002.J. Biol. Chem.277,7610.
Holinger, E.P., et al. 1999.J. Biol. Chem.274,13298.
Sui, D., and Wilson, J.E. 1997.Arch. Biochem. Biophys.345,111.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C188H291N53O40S2 |
| 分子量 | 3997.78 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | lyophilized solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze desiccated (hygroscopic) protect from light |
| 颜色 | white |
| 溶解性 | water: 1 mg/mL |
| 运输 | wet ice |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
