Calpain Substrate III, Fluorogenic-Calbiochem

半岛bd体育手机客户端 说明

一般描述

A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (k_cat/K_mx10^-2(M^-1s^-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and <0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC and α-spectrin cleavage site-based substrate. Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with higher efficiency (k_cat/K_mx10^-2(M^-1s^-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and <0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively). It is kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in intactDrosophilaS2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.

包装

1 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Product does not compete with ATP.
kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and <0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively
Primary Target
Substrate sequence that is optimized for calpain-1 and -2
Reversible: no
Cell permeable: no

警告

Toxicity: Irritant (B)

序列

DABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS

外形

Supplied as a trifluoroacetate salt.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Tompa, P., et al. 2004.J. Biol. Chem.279,20775.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C80H114N20O18S
分子量	1675.95

半岛bd体育手机客户端 性质

质量水平	100
测定	≥98% (HPLC)
形式	lyophilized solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze protect from light
颜色	red
溶解性	water: 1 mg/mL DMSO: 5 mg/mL
荧光	λex320 nm λem480 nm
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable