半岛bd体育手机客户端 说明
一般描述
A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported Kcat/kmvalues (min-1/?M) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).
A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. kcat/Kmvalues (min-1/?M) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 are 0.044 and 0.040, respectively.
包装
1 mg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Product does not compete with ATP.
Primary Target
β-secretase substrate
Reversible: no
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
外形
Supplied as a trifluoroacetate salt.
重悬
Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
其他说明
Ermolieff, J., et al. 2000.Biochemistry39,12450.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C91H129N25O25S |
| 分子量 | 2005.22 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | lyophilized solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze protect from light |
| 颜色 | red |
| 溶解性 | DMSO: 5 mg/mL |
| 荧光 | λex~350 nm λem~490 nm |
| 运输 | ambient |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
