Trypsin, Porcine, MS Grade

半岛bd体育手机客户端 说明

一般描述

A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 ?g of modified trypsin, and 1 ml of resuspension buffer.
A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry.

包装

1 set in Glass bottle

警告

Toxicity: Harmful (C)

单位定义

One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
One BTEE unit is defined as the amount of enzyme required to hydrolyze 1 ?mol BTEE per min at 25°C, pH 7.8

重悬

Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.

其他说明

Henzel, W.J., et al. 1993.PNAS90,5011.
Rice, R.H., et al. 1977.Biochim Biophys Acta492,316..

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
形式	lyophilized
specific activity	≥10,000 U/mg
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
异质活性	Chymostrypsin <3 BTEE units/mg solid
运输	wet ice
储存温度	?20℃