PP1 Analog II, 1NM-PP1-CAS 221244-14-0-Calbiochem

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一般描述

A cell-permeable and reversible PP1 analog (Cat. No. 529579) that acts as a potent, selective, and ATP-competitive inhibitor of mutant kinases compared to the corresponding wild-type kinase (IC₅₀= 3.2 nM for T339G, c-Fyn-as1 vs. 1.0 ?M for c-Fyn; 4.3 nM for I338G, v-src-as1 vs. 28 ?M for v-src; 5 nM for F80G, CDK2-as1 vs. 29 ?M for CDK2; 8 nM for F89G, CAMK IIα-as1 vs. 24 ?M for CAMKII; 120 nM for T315A, c-Abl-as2 vs. 3.4 ?M for c-Abl). Shown to activate mutants of Ire1, a transmembrane kinase.
A cell-permeable and reversible PP1 analog (Cat. No. 529579) that acts as a potent and selective ATP-competitive inhibitor of mutant kinases over wild-type (IC₅₀= 3.2 nM for T339G, c-Fyn-as1 vs. 1.0 ?M for c-Fyn; 4.3 nM for I338G, v-src-as1 vs. 28 ?M for v-src; 5 nM for F80G, CDK2-as1 vs. 29 ?M for CDK2; 8 nM for F89G, CAMK IIα-as1 vs. 24 ?M for CAMKII; 120 nM for T315A, c-Abl-as2 vs. 3.4 ?M for c-Abl). Shown to activate mutants of Ire1, a transmembrane kinase. Also available as a 10 mM solution in DMSO (Cat. No. 529606).

包装

1 mg in Glass bottle
Packaged under inert gas

生化/生理作用

Cell permeable: yes
Primary Target
T339G
Product competes with ATP.
Reversible: yes
Target IC₅₀: 3.2 nM for T339G, c-Fyn-as1 vs. 1.0 ?M for c-Fyn; 4.3 nM for I338G, v-src-as1 vs. 28 ?M for v-src; 5 nM for F80G, CDK2-as1 vs. 29 ?M for CDK2; 8 nM for F89G, CAMK IIα-as1 vs. 24 ?M for CAMKII; 120 nM for T315A, c-Abl-as2 vs.

警告

Toxicity: Standard Handling (A)

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他说明

Ira, G., et al. 2004.Nature431,1011.
Papa, F.R., et al. 2003.Science302,1533.
Bishop, A.C., et al. 2000.Nature407,395.
Weiss, E.L., et al. 2000.Nat. Cell Biol.2,677.
Bishop, A.C., et al. 1999.J. Am. Chem. Soc.121,627.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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