PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶EGF-treated HepG2 cells.
This PhosphoDetect Anti-GSK3β (pSer?) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer?).
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶EGF-treated HepG2 cells.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser? phosphorylation site of human glycogen synthase kinase 3β
Human

包装

1 set in Plastic ampoule

应用

ELISA (0.05 ?g/ml)

Immunoblotting (0.5 ?g/ml)

警告

Toxicity: Standard Handling (A)

外形

100 ?g antibody in PBS, PEG, sucrose, 50% glycerol and 200 ?l lyophilized control lysate.

重悬

Reconstitute the positive control lysate with 200 ?l H?O. Following complete solubilization, add 200 ?l SDS-PAGE sample buffer and incubate at 90°C for 5 min.

分析说明

Positive Control
Pervanadate-treated HepG2 (included)

其他说明

For immunoblotting with the positive control, use 10 ?l (chemiluminescence) or 20 ?l (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	2D3, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative (antibody only)
species reactivity	mouse, human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable