ERK Activation Inhibitor Peptide II, Cell-Permeable-Calbiochem

品牌

Sigma-Aldrich

货号

328005

包装型号

1 MG, The ERK Activation Inhibitor Peptide II, Cell-Permeable controls the biological activity of ERK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation application

规格纯度

The ERK Activation Inhibitor Peptide II, Cell-Permeable controls the biological activity of ERK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

价格

4137.78 元 *本价格含增值税费

促销

数量

- +

半岛bd体育手机客户端名称：

ERK Activation Inhibitor Peptide II, Cell-Permeable-Calbiochem
MTP _TAT-G-MEK1 ₁ ₃, H- GYGRKKRRQRRR-G-MPKKKPTPIQLNP-NH ₂, Erk Inhibitor V

半岛bd体育手机客户端介绍：

半岛bd体育手机客户端说明

一般描述

A cell-permeable and reversible 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK or MAPKK) that is fused to the HIV-TAT membrane translocation peptide (MTP) sequence via a glycine linker. Acts as a specific inhibitor of ERK activation by MEK1 and the downstream transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC₅₀= 210 nMin vitro). Potently blocks ERK in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC₅₀= 29 ?M). Does not inhibit the activation of c-jun N-terminal kinases (JNKs) or p38 MAP kinase.
A cell-permeable and reversible 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that is fused to the HIV-TAT membrane translocating peptide (MTP) sequence via a glycine linker. Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC₅₀= 210 nM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC₅₀= 29 ?M). Does not affect the activation of JNKs or p38.

包装

1 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Primary Target
MEK
Product does not compete with ATP.
Reversible: yes
Target IC₅₀: 210 nM against MEK; 29 ?M in blocking ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells
Cell permeable: yes

警告

Toxicity: Standard Handling (A)

序列

H-Gly-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH?

外形

Supplied as a trifluoroacetate salt.

重悬

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Kelemen, B.R., et al. 2002.J. Biol. Chem.277,8741.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C₁₃₆H₂₄₁N₅₃O₃₁S
分子量	3146.77

半岛bd体育手机客户端性质

质量水平	100
测定	≥95% (HPLC)
形式	lyophilized solid
manufacturer/tradename	Calbiochem^?
储存条件	OK to freeze desiccated (hygroscopic) protect from light
溶解性	water: 1 mg/mL
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable