Biotin, Fluorescein Conjugate

半岛bd体育手机客户端 说明

一般描述

Avidin (and streptavidin) has 4 binding sites for biotin. Taking advantage of the high affinity between avidin and biotin, this fluorescein-conjugated biotin-X (a 6-atom spacer) provides a method for fluorescent detection of biotinylated antibodies.

包装

1 mg in Plastic ampoule

应用

EIA (1:1,000)

Tissue Staining (1:50 - 1:100)

警告

Toxicity: Standard Handling (A)

重悬

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

其他说明

Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
Gruber, H.J., et al. 1998.Biochim. Biophys. Acta1381,203

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
形式	lyophilized solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze protect from light
颜色	orange
溶解性	DMF: 5 mg/mL DMSO: 5 mg/mL methanol: 5 mg/mL
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable