半岛bd体育手机客户端 说明
一般描述
GranuCult?Half FRASER broth (base) with antibiotics has high nutrient content and a large buffering capacity that creates optimum growth conditions forListeriaspp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride inhibit the growth of accompanying bacteria. The detection of the β-D-glucosidase activity is possible by adding aesculin and ammonium iron (III) citrate, forming a black complex of aesculin with iron (III) ions. A black coloration can develop during incubation, but this reaction is not exclusive toListeriaspp. Hence, according to EN ISO 11290-1, every primary and secondary enrichment in Fraser broth must be sub-cultured on selective plating media. From TM="GranuCult"] Half FRASER (Demi FRASER) Broth (Base) with Antibiotics primary selective enrichment Half Fraser broth can be prepared by addition of one vial Ammonium iron (III) citrate supplement (1.00092) per 500 mL Half Fraser Broth (base). For the secondary enrichment step, Fraser broth can be prepared by adding one additional vial of reconstituted FraserListeriaselective supplements (1.00093) per 500 mL Half Fraser Broth.
应用
GranuCult?Half FRASER broth (base) with antibiotics is suitable for the primary selective enrichment ofListeriaspp. from food and animal feed as well as from environmental samples and other materials.
分析说明
Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Afterwards confirmation on ALOA?-Medium resp. Tryptic Soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Growth on ALOA
Growth on ALOA
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)): blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)): blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <100 colonies
Incubation:
Half Fraser Broth: 24 ± 2 hours at 30 ± 1°C
Confirmation on ALOA
Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1°C
附注
We offer two media types: the superior granulated GranuCult?and the cost-efficient powdered NutriSelect?culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
ALOA is a registered trademark of Biolife Italiana S.r.l.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| agency | APHA according to ISO 11290 |
| 形式 | medium granules (dehydrated (DCM)) |
| 包装 | pkg of 500 g |
| manufacturer/tradename | GranuCult? |
| 储存条件 | protect from light (both UV light and sun light) |
| technique(s) | microbiological culture: suitable |
| pH值(酸碱度) | 7.0-7.4 (25 ℃, 55 g/L in H2O, after autoclaving) |
| 溶解性 | 55 g/L |
| application(s) | food and beverages microbiology pathogen testing |
| 储存温度 | 15-25℃ |
| 适用性 | Listeriaspp. |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
Sigma-Aldrich
m.cnreagent.com
