Gram′s crystal violet solution

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一般描述

Gram′s crystal violet solution for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218), an aniline dye. After the treatment with iodine solution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, Product number 1.10218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Gram′s safranine solution (Product number 1.09217) and will then appear pink to red.
A bottle with 500 ml Gram′s crystal violet solution is sufficient for staining up to 250 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

分析说明

Suitability for microscopy: passes test
gram-positve microorganisms: blue-violet
gram-negative microorganisms: pink to red

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安全信息

象形图
警示用语：	Warning
危险声明	H226 - H411
预防措施声明	P210 - P233 - P240 - P241 - P242 - P273
危险分类	Aquatic Chronic 2 - Flam. Liq. 3
储存分类代码	3 - Flammable liquids
WGK	WGK 3
闪点(F)	116.6 °F
闪点(C)	47 ℃