Readycult^?Coliforms 100

半岛bd体育手机客户端 说明

一般描述

Coliforms 100 is a chromogenic medium that provides easy-to-read, definitive positive results. It has a high nutritional quality of the peptones along with incorporated phosphate buffer that guarantees rapid growth of coliforms, while lauryl sulfate largely inhibits the accompanying flora (especially the Gram-positive). The chromogenic substrates X-GAL (cleaved by coliforms) and the fluorogenic substrate MUG (highly specific forE. coli) allow for the simultaneous detection of total coliforms andE. coli. The presence of total coliforms is indicated by a blue-green color of the broth andE. coliby a blue fluorescence under UV-light. The optional 30-second indole reaction helps to accurately confirm the presence ofE. coli. ReadyCult^?tests are convenient, rapid and economical.

应用

Coliforms 100 is a rapidE. coli/Coliform test for drinking water. It is a selective enrichment broth used for the simultaneous presence / absence detection of total coliforms andE. coliwithin the bacteriological water examination.

联系

Replaces: 1012980002

分析说明

Appearance
- clearness:
clear
- colour: yellowish
pH-value (25?°C): 6.6 - 7.0
Inoculum on reference medium
- Escherichia coli ATCC 11775 (WDCM 00090):
10 - 100
- Citrobacter freundii ATCC 8090: 10 - 100
- Klebsiella pneumoniae ATCC 31488: 10 - 100
- Salmonella typhimurium ATCC 14028 (WDCM 00031): 10 - 100
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): ≥ 1000
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): +
colour change to blue-green
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Fluorescence
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Indole formation
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
Incubation: 24 hrs.; 35 °C; aerobic.

法律信息

READYCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	200
agency	EPA (Approved as alternative method)
无菌性	sterile; γ-irradiated
形式	medium granules (dehydrated (DCM))
manufacturer/tradename	ReadyCult?
technique(s)	microbiological culture: suitable
pH值(酸碱度)	6.6-7.0 (25 ℃, 17 g/L in H2O)
溶解性	17 g/L
application(s)	food and beverages microbiology
储存温度	15-25℃
适用性	Escherichia coli coliforms

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 2