GranuCult^?Baird-Parker agar (base)

半岛bd体育手机客户端 说明

一般描述

GranuCult^?Baird-Parker agar (base) is used for the isolation and enumeration ofStaphylococcus aureus. This medium contains lithium chloride and tellurite to inhibit the growth of accompanying microbial flora, whereas pyruvate and glycine selectively stimulate the growth of staphylococci. Agar is the solidifying agent. The egg-yolk content makes the medium opaque.Staphylococcuscolonies show two characteristic features when grown in this medium: (a) characteristic zones and rings are formed as a result of lipolysis and proteolysis, and (b) reduction of tellurite to tellurium produces a black coloration. The egg-yolk reaction and tellurite reduction usually occur together with a positive coagulase reaction and thus can serve as an index for the latter. According to EN ISO 6888-1, the addition of sulfamethazine is advised to suppress the growth and swarming ofProteussp., if these are suspected in the test sample.

应用

GranuCult^?Baird-Parker agar (base) is used for the isolation, presumptive identification, and enumeration ofStaphylococcus aureusfrom food and animal feed as well as from other materials.

联系

Replaces: 1.05406.5007

分析说明

Appearance (clearness): clear to turbid
Appearance (color): yellowish-brown
pH-value (25?°C): 6.6 - 7.0
Solidification behaviour (2?hrs., 45?°C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 50 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Staphylococcus saprophyticus ATCC 15305 (WDCM 00159)): no limit
Growth (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): no limit
Growth (Enterococcus hirae ATCC 8043 (WDCM 00089)): no limit
Growth (Proteus mirabilis ATCC 29906 (WDCM 00023)): no limit
Colonies (Staphylococcus aureus ATCC 6538 (WDCM 00032)): Black or grey colonies with clear halo
Colonies (Staphylococcus aureus ATCC 25923 (WDCM 00034)): Black or grey colonies with clear halo
Colonies (Staphylococcus saprophyticus ATCC 15305 (WDCM 00159)): Black or grey colonies without clear halo
Colonies (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): Black or grey colonies without clear halo
Colonies (Enterococcus hirae ATCC 8043 (WDCM 00086)): Black or grey colonies without clear halo
Colonies (Proteus mirabilis ATCC 29906 (WDCM 00023)): Brown or black colonies without clear halo
Incubation:
24 ± 2 to 48 ± 2 hours at 37 ± 1 °C aerobic
E.coli 48 ± 2 hours at 37 ± 1 °C aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

附注

We offer two media types: the superior granulated GranuCult^?and the cost-efficient powdered NutriSelect^?culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	200
agency	APHA ISO 6888
reg. compliance	FDA (BAM)
形式	medium granules (dehydrated (DCM))
manufacturer/tradename	GranuCult?
technique(s)	microbe id | specific enzyme detection: suitable microbiological culture: suitable
pH值(酸碱度)	6.8 (37 ℃, 58 g/L in H2O)
溶解性	58 g/L
堆积密度	570 kg/m3
application(s)	food and beverages microbiology
储存温度	15-25℃
适用性	Staphylococcusspp.

安全信息

象形图
警示用语：	Warning
危险声明	H317 - H319
预防措施声明	P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Skin Sens. 1
储存分类代码	11 - Combustible Solids
WGK	WGK 1