Chromocult^?Tryptone Bile X-glucuronide agar

半岛bd体育手机客户端 说明

一般描述

Chromocult^?Tryptone Bile X-glucuronide agar is a selective medium for the enumeration ofEscherichia coli. The presence of the enzyme β-D-glucuronidase differentiates mostE. colifrom other coliforms.E. coliabsorbs the chromogenic substrate 5-bromo-4-chloro-3- indolyl-β-D-glucuronide (X-β-D-glucuronide) while, the enzyme β-glucuronidase splits the bond between the chromophore 5-bromo-4-chloro-3-indolyl and the β-D-glucuronide. The formedE. colicolonies are colored blue to blue green. The growth of accompanying Gram-positive flora is largely inhibited due to the presence of bile salts and the high incubation temperature of 44℃. SomeE. coli(3-4 %) are β-D-glucuronidase negative and appear as colorless colonies (e.g. mostE. coliO157 strains) or do not grow at the elevated temperature of 44℃ (e.g.E. coli0157:H7).

应用

Chromocult^?Tryptone Bile X-glucuronide agar is used for the enumeration, detection, and identification of β-glucuronidase positiveEscherichia colifrom food and feeding stuff, as well as from environmental samples in the area of food production and food handling.

分析说明

Appearance (clearness): clear
Appearance (colour): yellowish to yellowish-brown
pH-value (25?°C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium(Escherichia coli NCTC 13216 (WDCM00202)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Escherichia coli NCTC 13216 (WDCM00202)):
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 50 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 50 %
Recovery on test medium(Escherichia coli NCTC 13216 (WDCM00202)): ≥ 50 %
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Growth (Citrobacter freundii ATCC 43864 (WDCM 00006)): no limit
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): turquoise to blue
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): turquoise to blue
Colonies ((Escherichia coli NCTC 13216 (WDCM00202)): turquoise to blue
Colonies (Citrobacter freundii ATCC 43864 (WDCM 00006)): white to green-beige
Colonies (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): white to green-beige
Incubation: 21 ± 3 hours at 44 ± 1 °C aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

法律信息

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	200
agency	ISO 16649
形式	medium granules (dehydrated (DCM))
manufacturer/tradename	ChromoCult?
technique(s)	microbiological culture: suitable
pH值(酸碱度)	7.0-7.4 (25 ℃, 36.6 g/L in H2O, after autoclaving)
溶解性	31.6 g/L
application(s)	food and beverages microbiology
储存温度	15-25℃
适用性	Escherichia coli

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3