半岛bd体育手机客户端 说明
一般描述
Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.
应用
Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It has also been used in Bradford protein assay.
分析说明
鉴定标准(UV/VIS-光谱)：通过测试
最大吸光值λ最大(缓冲液pH7.0)：577 - 584 nm
光谱吸光性A 1%/1cm(λ最大；0.01?g/l；缓冲液pH?7.0；基于干物质计算)：450 - 570
TLC-测试：通过测试
干燥损失(100?°C)： ≤ 8 %
电泳适用性：通过测试
法律信息
null
基本信息
| 经验(实验)分子式 | C47H48N3NaO7S2 |
| 分子量 | 854.02 |
| MDL编号 | MFCD00078482 |
| EC索引号 | 228-058-4 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 效能 | >5000 mg/kg LD50, oral (Rat) |
| technique(s) | protein staining: suitable |
| pH值(酸碱度) | 6.4 (20 ℃, 10 g/L in H2O) |
| 溶解性 | 40 g/L |
| 堆积密度 | 520 kg/m3 |
| 储存温度 | no temp limit |
| InChI | 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1 |
| InChI key | CMGWKYXJIYYESN-UHFFFAOYSA-M |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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