Olig2 (211F1.1) Mouse Monoclonal Antibody

浜у搧璇存槑

涓€鑸弿杩?/p>

Olig2, a basic helix鈥搇oop鈥揾elix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuroepithelial tumors, ependymomas, subependymomas, medulloblastomas, and nonneuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid/rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis). In order to characterize cellular subtypes that constitute astrocytomas, oligoastrocytomas and oligodendrogliomas, double labeling of Olig2 and GFAP has been performed which identified two phenotypically distinct tumor populations. The first is Olig2+/GFAP鈥 which has an oligodendroglial morphology, corresponding to pure oligodendrogliomas that contain only oligodendroglial cells; the second is Olig2鈥?GFAP+ which has an astrocytic phenotype, including not only oligoastrocytomas, but also WHO astrocytomas. Depending on proportion and spatial clustering of the two phenotypically distinct tumor populations, the tumor (Olig2-/GFAP+) is classified either as an astrocytoma when both populations are intermingled with a dominance of GFAP+ cells or oligoastrocytoma when there is some degree of spatial clustering of the GFAP+ cells.

璐ㄩ噺

IVD

鑱旂郴

Olig2 Positive Control Slides, Product No. 387S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

澶栧舰

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

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For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

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Cell Marque is a trademark of sigma-aldrich Co. LLC

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NACRES

NA.41

浜у搧鎬ц川

璐ㄩ噺姘村钩	500 100
鐢熺墿鏉ユ簮	mouse
鍋惰仈鐗?/td>	unconjugated
鎶椾綋褰㈠紡	culture supernatant
antibody product type	primary antibodies
鍏嬮殕	211F1.1, monoclonal
鎻忚堪	For In Vitro Diagnostic Use in Select Regions (See Chart)
褰㈠紡	buffered aqueous solution
species reactivity	human
鍖呰	vial of 0.1 mL concentrate (387M-14) vial of 0.5 mL concentrate (387M-15) bottle of 1.0 mL predilute (387M-17) vial of 1.0 mL concentrate (387M-16) bottle of 7.0 mL predilute (387M-18)
manufacturer/tradename	Cell Marque^?
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
鍚屼綅绱?浜氬瀷	IgG2aκ
鎺у埗	astrocytoma
杩愯緭	wet ice
鍌ㄥ瓨娓╁害	2-8鈩傸/td>
鍙鍖朁/td>	nuclear