Glutamine Synthetase (GS-6) Mouse Monoclonal Antibody

浜у搧璇存槑

涓€鑸弿杩?/p>

Glutamine synthetase (GS) catalyzes the synthesis of glutamine from glutamate and ammonia in the mammalian liver.1 In normal liver, GS expression is seen in pericentral hepatocytes, but not in mid-zonal or periportal hepatocytes.1 Glutamine, the end product of GS activity, is the major energy source of tumor cells.1 Based on findings from experimental hepatocarcinogenesis, GS positive tumor cells are believed to be derived from GS positive hepatocytes.1 Thus, anti-GS has been suggested as a marker for hepatocellular carcinoma (HCC).1 GS immunoreactivity has been seen in a majority of HCC (37 of 53 cases, 70%), including 7 of 10 cases of early HCC (70%) and 12 of 22 (59%) for low grade HCC.1 In nonmalignant nodules, GS overexpression was only seen in 3 high grade dysplastic nodules (HGDN,13.6%). In these cases, GS overexpression was restricted to 11.5%-50% of hepatocytes, whereas in HCC the majority of cases (28 of 53, 53%), including early HCC (60%), showed diffuse immunostaining (>50% tumor cells). Overall, the sensitivity, specificity, and positive and negative predictive values of anti-GS for HCC detection were 69.8%, 94.2%, 92.5%, and 75.4%, respectively. A panel composed of antibodies against HSP70, GPC3, and GS has been proposed to be very useful in distinguishing between dysplastic and early malignant hepatocellular nodules arising in cirrhosis. The 鈥渁ll positive鈥 phenotype is restricted to approximately half of early HCC to well-differentiated HCC but has never been reported in dysplastic lesions, whereas the reverse phenotype, 鈥渁ll negative鈥? has been shown to be a feature of the majority of HGDN and of all low grade dysplastic nodules.

璐ㄩ噺

IVD

RUO

鑱旂郴

Glutamine Synthetase Positive Control Slides, Product No. 389S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

澶栧舰

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

鍒跺璇存槑

Download the IFU specific to your product lot and format
Note: This requires a keycode which can be found on your packaging or product label.

Download the latest released IFU
Note: This IFU may not apply to your specific product lot.

鍏朵粬璇存槑

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

鍩烘湰淇℃伅

NACRES

NA.41

浜у搧鎬ц川

璐ㄩ噺姘村钩	500 100
鐢熺墿鏉ユ簮	mouse
鍋惰仈鐗?/td>	unconjugated
鎶椾綋褰㈠紡	culture supernatant
antibody product type	primary antibodies
鍏嬮殕	GS-6, monoclonal
鎻忚堪	For In Vitro Diagnostic Use in Select Regions (See Chart)
褰㈠紡	buffered aqueous solution
species reactivity	human
鍖呰	vial of 0.1 mL concentrate (389M-14) vial of 0.5 mL concentrate (389M-15) bottle of 1.0 mL predilute (389M-17) vial of 1.0 mL concentrate (389M-16) bottle of 7.0 mL predilute (389M-18)
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
鍚屼綅绱?浜氬瀷	IgG2a
鎺у埗	hepatocellular carcinoma
杩愯緭	wet ice
鍌ㄥ瓨娓╁害	2-8鈩傸/td>
鍙鍖朁/td>	cytoplasmic