Anti-S-100 Protein Antibody, clone 15E2E2

浜у搧璇存槑

涓€鑸弿杩?/p>

S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.

鐗瑰紓鎬?/p>

Recognizes intact, alpha and beta subunits of bovine, human, and murine S-100 protein, as determined by immunoprecipitation profiles.

鍏嶇柅鍘烖/p>

Purified bovine S-100 protein
Epitope: Recognizes both alpha & beta S100

搴旂敤

Research Sub Category
Muscle Physiology
Research Category
Metabolism
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
Anti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB.

璐ㄩ噺

Evaluated by Western Blot on PC12 lysates
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 ?g of PC12 lysate.

澶栧舰

Purified mouse monoclonal IgG2aκ in buffer containing PBS, pH 7.6, 1% BSA and 0.09% sodium azide.
Format: Purified
Protein A purified

鍌ㄥ瓨鍙婄ǔ瀹氭€?/p>

Stable for 1 year at 2-8?C from date of receipt.

鍒嗘瀽璇存槑

Control
Positive Control Tissue: Melanoma

鍏朵粬璇存槑

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

娉曞緥淇℃伅

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

鍏嶈矗澹版槑

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

鍩烘湰淇℃伅

eCl@ss	32160702
NACRES	NA.41

浜у搧鎬ц川

璐ㄩ噺姘村钩	300 100
鐢熺墿鏉ユ簮	mouse
鎶椾綋褰㈠紡	purified immunoglobulin
antibody product type	primary antibodies
鍏嬮殕	15E2E2, monoclonal
species reactivity	rat, human, bovine, mouse
manufacturer/tradename	Chemicon^?
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable western blot: suitable
鍚屼綅绱?浜氬瀷	IgG2aκ
NCBI鐧昏鍙饵/td>	NM_006271.1
UniProt鐧昏鍙饵/td>	P23297
杩愯緭	wet ice