鎷瓙鐥呮瘨閲囨牱鍙婅繍杈撹瘯鍓傜洅锛屽吋瀹圭粏鑳炲煿鍏伙紙宸插垎瑁咃級璁捐寮曠墿搴旈伒寰互涓嬪師鍒欙細
鈶犲紩鐗╅暱搴︼細 15-30bp锛屽父鐢ㄤ负20bp宸﹀彸銆侟/span>

鈶″紩鐗╂墿澧炶法搴︼細 浠?00-500bp涓哄疁锛岀壒瀹氭潯浠朵笅鍙墿澧為暱鑷?0kb鐨勭墖娈点€侟/span>

鈶㈠紩鐗╃⒈鍩猴細G+C鍚噺浠?0-60%涓哄疁锛孏+C澶皯鎵╁鏁堟灉涓嶄匠锛孏+C杩囧鏄撳嚭鐜伴潪鐗瑰紓鏉″甫銆侫TGC濂介殢鏈哄垎甯冿紝閬垮厤5涓互涓婄殑鍢屽懁鎴栧槯鍟舵牳鑻烽吀鐨勬垚涓叉帓鍒椼€侟/span>

鈶ｉ伩鍏嶅紩鐗╁唴閮ㄥ嚭鐜颁簩绾х粨鏋勶紝閬垮厤涓ゆ潯寮曠墿闂翠簰琛ワ紝鐗瑰埆鏄?鈥欑鐨勪簰琛ワ紝鍚﹀垯浼氬舰鎴愬紩鐗╀簩鑱氫綋锛屼骇鐢熼潪鐗瑰紓鐨勬墿澧炴潯甯︺€侟/span>

鈶ゅ紩鐗?鈥欑鐨勭⒈鍩猴紝鐗瑰埆鏄痾ui鏈強鍊掓暟绗簩涓⒈鍩猴紝搴斾弗鏍艰姹傞厤瀵癸紝浠ラ伩鍏嶅洜鏈纰卞熀涓嶉厤瀵硅€屽鑷碢CR澶辫触銆侟/span>

鈶ュ紩鐗╀腑鏈夋垨鑳藉姞涓婂悎閫傜殑閰跺垏浣嶇偣锛岃鎵╁鐨勯澏搴忓垪zui鏈夐€傚疁鐨勯叾鍒囦綅鐐癸紝杩欏閰跺垏鍒嗘瀽鎴栧垎瀛愬厠闅嗗緢鏈夊ソ澶勩€侟/span>

鈶﹀紩鐗╃殑鐗瑰紓鎬э細寮曠墿搴斾笌鏍搁吀搴忓垪鏁版嵁搴撶殑鍏跺畠搴忓垪鏃犳槑鏄惧悓婧愭€с€侟/span>
瀹為獙娉ㄦ剰浜嬮」锛欬/span>

1锛塕T-PCR鍙互妫€娴嬬粍缁囥€佺粏鑳炪€佽娑层€佺粏鑿岀瓑寰堝鏉愭枡锛屼笉鍚岀殑鏍锋湰鏈変笉鍚岃姹傦紝瀹為獙鍓嶈鍏呭垎娌熼€氱‘璁ゅ疄楠屾柟妗堝拰鏍锋湰鎯呭喌锛汓/span>

2锛夊鎴峰敖鍙兘鎻愪緵瀹為獙鐨勮儗鏅俊鎭€佺墿绉嶃€佸熀鍥犲噯纭殑鍚嶇О鍜孖D鍙凤紱

3锛夊鎴峰敖閲忎笉瑕佹彁渚汥NA鎴朢NA鏍峰搧銆侟/span>

4锛夋牱鏈繚瀛樹簬娑叉爱鎴栧共鍐帮紝涔熷彲浠ヤ繚瀛樹簬Trizol娑蹭腑銆侟/span>

PCR鍙嶅簲浜旇绱狅細

鍙傚姞PCR鍙嶅簲鐨勭墿璐ㄤ富瑕佹湁浜旂鍗冲紩鐗┿€侀叾銆乨NTP銆佹ā鏉垮拰Mg2+

寮曠墿锛欬/span>寮曠墿鏄?/span>PCR鐗瑰紓鎬у弽搴旂殑鍏抽敭锛孭CR 浜х墿鐨勭壒寮傛€у彇鍐充簬寮曠墿涓庢ā鏉緿NA浜掕ˉ鐨勭▼搴︺€傜悊璁轰笂锛屽彧瑕佺煡閬撲换浣曚竴娈垫ā鏉緿NA搴忓垪锛屽氨鑳芥寜鍏惰璁′簰琛ョ殑瀵℃牳鑻烽吀閾惧仛寮曠墿锛屽埄鐢≒CR灏卞彲灏嗘ā鏉緿NA鍦ㄤ綋澶栧ぇ閲忔墿澧炪€侟/span>

浜у搧鍚嶇О锛欬/span>鎷瓙鐥呮瘨閲囨牱鍙婅繍杈撹瘯鍓傜洅锛屽吋瀹圭粏鑳炲煿鍏伙紙宸插垎瑁咃級

鑻辨枃鍚嶇О锛氳瑙佽鏄庝功

缂栧彿锛欬/span>

鍒嗙被锛欬/span>PCR妫€娴嬭瘯鍓傜洅

鍌ㄥ瓨鏉′欢锛欬/span>-20鈩冮伩鍏変繚瀛橈紝閬垮厤鍙嶅鍐昏瀺銆侟/span>

杩愯緭锛氫綆娓┿€侀伩鍏夈€侟/span>

鍑嗗鐗╁搧锛欬/span>

娓呯悊娑诧紙A锛?nbsp; 姣崌

鏌撹壊娑诧紙t B锛?nbsp; 寰崌

绋€閲婃恫锛圏/span>C锛?nbsp; 姣崌

婧惰В娑诧紙tD锛?nbsp; 姣崌

浜у搧璇存槑涔?/span> 1浠键/span>

瀹冨叿鏈変笅鍒楃壒鐐癸細

1. 涓€绠″紡鎿嶄綔锛岀敤鎴峰彧闇€瑕佹彁渚涙牱鍝佸嵆鍙€侟/span>

2. 鏍规嵁澶ц眴鐑紤鍏嬭泲鐧藉熀鍥犱繚瀹堝尯鍩熻璁″紩鐗╋紝鑳戒笓涓€鎬ф娴嬪嚭鏍峰搧涓殑澶ц眴鎴愬垎锛屼絾涓嶈兘妫€娴嬪叾浠栭潪澶ц眴鎴愬垎銆侟/span>

3. 蹇€燂紝鏁翠釜妫€娴嬭繃绋嬶紙鎸変竴涓牱鍝佽锛夋墍闇€鏃堕棿浠呬负 2.0 灏忔椂銆侟/span>

4. 鍙渶瑕佹櫘閫 PCR 浠拰鍑濊兌鐢垫吵浠紝鏃犻渶閰嶇疆璐甸噸浠櫒璁惧銆侟/span>

5. 瀵规贩鍚堟牱鍝佷腑澶ц眴鎴愬垎鐨勬娴嬩笅闄愪负 0.1%锛屽鏍峰搧涓ぇ璞嗘垚鍒嗙殑鏍搁吀妫€娴嬩笅闄愪负 1.0ng/渭L銆侟/span>

6. 鏈彧鑳界敤浜庣鐮旓紝瓒冲 50 娆 40uL 浣撶郴鐨勫父瑙 PCR銆侟/span>

MIF 宸ㄥ櫖缁嗚優绉诲姩鎶戝埗鍥犲瓙鎶椾綋榛勮姫鐨傝嫹III

JIP1/MAPK8IP1 涓濊鍘熸椿鍖栬泲鐧芥縺閰?鐩镐簰浣滅敤铔嬬櫧1榛勮姫鐨傝嫹II

MIP-1 Alpha 宸ㄥ櫖缁嗚優鐐庣棁鍥犲瓙1伪鎶椾綋 MIP-1伪榛勬潪鑻饵/span>

MIP-1 Beta/CCL4 宸ㄥ櫖缁嗚優鐐庣棁鍥犲瓙1尾 鎶椾綋 MIP-1尾榛勮棨绱燑/span>

MIP4/CCL18 宸ㄥ櫖缁嗚優鐐庣棁铔嬬櫧18鎶椾綋榛勬潹閰⒈

MITF MITF鐩稿叧杞綍鍥犲瓙鎶椾綋鑼磋姽鍐呴叝

MMP-1 鍩鸿川閲戝睘铔嬬櫧閰?1鎶椾綋绉洩鑽夐吀

MMP-1 鍩鸿川閲戝睘铔嬬櫧閰?1鎶椾綋鍚夐┈閰?/span>

MMP-2 鍩鸿川閲戝睘铔嬬櫧閰?2鎶椾綋鐢插熀鑾插績纰包/span>

MMP-3 鍩鸿川閲戝睘铔嬬櫧閰?鎶椾綋鐢插３绱燑/span>
鎷瓙鐥呮瘨閲囨牱鍙婅繍杈撹瘯鍓傜洅锛屽吋瀹圭粏鑳炲煿鍏伙紙宸插垎瑁咃級OAT-1/SLC22A6 闃寸瀛愯浆杩愯泲鐧?1鎶椾綋鏌磋儭鐨傝嫹A

OAT-1/SLC22A6 闃寸瀛愯浆杩愯泲鐧?1鎶椾綋鏌磋儭鐨傝嫹B2

OAT-3 闃寸瀛愯浆杩愯泲鐧?3鎶椾綋鏌磋儭鐨傝嫹C

OAT4L/URAT1 灏块吀鐩愰噸鍚告敹杞繍瀛?鎶椾綋鏌磋儭鐨傝嫹D

Ghrelin 28 鑴戣偁鑲芥姉浣撹熅姣掔伒

OCLN/Occludin 绱у瘑杩炴帴铔嬬櫧鎶椾綋锜炬瘨浠栫伒

Claudin 5 绱у瘑杩炴帴铔嬬櫧5鎶椾綋闀挎槬鑳裹/span>

OCT1 闃崇瀛愯浆杩愬櫒1鎶椾綋闀挎槬鍦拌緵

OCT2 闃崇瀛愯浆杩愯泲鐧?鎶椾綋闀挎槬鑺辩⒈

Oct-1 鑳氳儙骞茬粏鑳炲叧閿泲鐧?鎶椾綋闀挎槬鏂扮⒈
瀹炴椂鑽у厜瀹氶噺PCR锛坬uantitative real-time PCR锛宷PCR锛夋槸鎸囧湪PCR鍙嶅簲浣撶郴涓姞鍏ヨ崸鍏夊熀鍥紝鍒╃敤鑽у厜淇″彿绉疮瀹炴椂鐩戞祴鏁翠釜PCR杩涚▼锛屾渶鍚庨€氳繃鏍囧噯鏇茬嚎瀵规湭鐭ユā鏉胯繘琛屽畾閲忓垎鏋愮殑鏂规硶銆傚疄鏃惰崸鍏夊畾閲廝CR鐨勫寲瀛﹀師鐞嗗寘鎷帰閽堢被鍜岄潪鎺㈤拡绫讳袱绫伙紝鎺㈤拡绫绘槸鍒╃敤涓庨澏缁嗚優搴忓垪鐗瑰紓鎬ф潅浜ょ殑鎺㈤拡鏉ユ寚绀烘墿澧炰骇鐗╃殑澧炲姞锛岄潪鎺㈤拡绫绘槸鍒╃敤鑽у厜鏌撴枡鎴栬€呯壒寮傛€ц璁＄殑寮曠墿鏉ユ寚绀烘墿澧炵殑澧炲姞銆傝繍鐢ㄨ鎶€鏈彲浠ュDNA銆丷NA鏍峰搧杩涜瀹氶噺锛堝寘鎷粷瀵瑰畾閲忓拰鐩稿瀹氶噺锛夊拰瀹氭€у垎鏋愩€侟/span>

涓昏鏈嶅姟锛欬/span>DNA鎴朢NA鐨勭粷瀵瑰畾閲忓垎鏋愩€佸熀鍥犺〃杈惧樊寮傚垎鏋愩€佸熀鍥犲垎鍨嬨€侟/span>

涓昏鎶€鏈細寮曠墿璁捐銆?/span>RNA鎻愬彇銆乧DNA鍚堟垚銆乹PCR銆侟/span>