鏌撴枡娉曢功楣夌儹琛ｅ師浣撳疄鏃跺畾閲廝CR璇曞墏鐩扅/span>

Dye-quantitative Real-time PCR Kit forChlamydia psittaci

璐у彿锛欳qs-psi-20 瑙勬牸锛?0涓弽搴擖/span>

璐у彿锛欳qs-psi-50 瑙勬牸锛?0涓弽搴擖/span>

璐у彿锛欳qs-psi-100 瑙勬牸锛?00涓弽搴擖/span>

鍌ㄥ瓨锛?20搴︼紝閬垮厜淇濆瓨锛屾湁鏁堟湡涓€骞淬€傞伩鍏嶅弽澶嶅喕铻嶃€侟/span>

璇曞墏鐩掔壒鐐癸細

1锛?nbsp; 鐗瑰紓鎬ф娴嬮功楣夌儹琛ｅ師浣擄紝涓庡叾浠栫敓鐗╁熀鍥犵粍鏃犱氦鍙夊弽搴斻€侟/span>

2锛?nbsp; 鐏垫晱搴︽槑鏄鹃珮浜庤娓呭鏂规硶锛屽ぇ绾﹂珮浜庢櫘閫歅CR娉曞崄鍊嶃€侟/span>

3锛?nbsp; DNA鑱氬悎閰堕噰鐢ㄧ儹鍚姩鏂瑰紡锛屽彲鎶戝埗闈炵壒寮傛€ф墿澧烇紝闄嶄綆鑳屾櫙鑽у厜銆侟/span>

4锛?nbsp; 甯︽湁闃虫€у鐓ф牱鍝侊紝鍙敤浜庢楠岃瘯鍓傜洅鏈夋晥鎬с€侟/span>

5锛?nbsp; 甯︽湁UDG閰跺拰dUTP锛屽彲闄嶄綆娈嬬暀DNA鐨勬薄鏌撱€侟/span>

6锛?nbsp; 甯︽湁ROX鍙傛瘮鏌撴枡锛屽府鍔╂牎姝ｅ姞鏍疯宸拰绠￠棿宸紓銆侟/span>

楣﹂箟鐑。鍘熶綋锛圏em>Chlamydia psittaci锛夛紝鎴栫О楣﹂箟鐑棞琛ｅ師浣?楣﹂箟鐑翰琛ｅ師浣擄紙Chlamydophila psittaci锛夈€侀功楣夎。鍘熶綋锛屾槸涓€绉嶄弗鏍肩粏鑳炲唴瀵勭敓鐨勫師鏍哥敓鐗╋紝灞炰簬琛ｅ師浣撶锛圏em>Chlamydiaceae锛夈€傞功楣夌儹琛ｅ師浣撴槸鏈€鏃╁彂鐜扮殑琛ｅ師浣擄紝瀹夸富闈炲父骞挎硾锛屼笉浠呮湁鍖呮嫭楣﹂箟鍦ㄥ唴鐨勫绉嶉笩绫伙紝杩樻湁璁稿鍝轰钩鍔ㄧ墿锛涘湪璁稿瀹夸富涓凡褰㈡垚鐙壒鐨勪簹鍨嬶紝閮ㄥ垎浜氬瀷宸茶鍒掑垎涓虹嫭绔嬬墿绉嶏紝濡傦細鍏界被琛ｅ師浣擄紙Chlamydia pecorum锛夈€佹祦浜ц。鍘熶綋锛圏em>Chlamydia abortus锛夈€佽睔榧犺。鍘熶綋锛圏em>Chlamydia caviae锛夈€佺尗琛ｅ師浣擄紙Chlamydia felis锛夌瓑銆傞功楣夌儹琛ｅ師浣撶殑浼犳挱鍙€氳繃鐩存帴鎺ヨЕ銆侀笩绮€侀蓟鍒嗘硨鐗╁拰姘旀憾鑳讹紝寮曡捣楦熺被鍜屽摵涔冲姩鐗╃殑鍛煎惛閬撶柧鐥咃紱鐢变簬鍙湪鐜涓繚鎸佹劅鏌撳姏杈炬暟鏈堜箣涔咃紝鍥犳鍙互閫犳垚鍏叡瀹夊叏濞佽儊锛屽寘鎷湪瀹剁鍏绘畺涓殑澶ц妯＄垎鍙戯紝浠ュ強鍦ㄤ汉绫讳腑鍋跺皵寮曞彂鐨勯功楣夌梾锛堟垨绉伴笩鐤€侀功楣夌儹锛夈€備汉绫讳竴鑸槸浠庡鍏绘垨閲庣敓楦熺被鑾峰緱鎰熸煋锛屼汉涓庝汉涔嬮棿鐨勪紶鎾皻寰呰瘉瀹烇紝浜虹被鎰熸煋鍚庡父娌℃湁鐥囩姸鎴栧嚭鐜扮被浼兼祦鎰熺殑鐥囩姸锛屾湁鏃朵細鍙戝睍涓轰弗閲嶈偤鐐庛€佸績鍐呰啘鐐庢垨鑴戠値銆傞功楣夌儹琛ｅ師浣撳垎涓哄緢澶氬熀鍥犲瀷锛屽寘鎷細A鈥揊, M56鍜學C锛屾瘡绉嶄簹鍨嬮兘鍏锋湁涓ユ牸鐨勫涓荤壒寮傛€э細A浜氬瀷瀹夸富涓洪功楣夌楦熺被锛孊浜氬瀷瀹夸富涓洪附锛孋浜氬瀷瀹夸富涓洪腑鍜岄箙锛孌浜氬瀷瀹夸富涓虹伀楦★紝E浜氬瀷瀹夸富涓洪附銆侀腑鍙婂叾浠栭笩绫伙紝F浜氬瀷瀹夸富涓洪功楣夛紝WC浜氬瀷瀹夸富涓虹墰锛孧56浜氬瀷瀹夸富鍒欎负鍟娇绫诲姩鐗┿€侟/span>

楣﹂箟鐑。鍘熶綋鍙娇鐢ㄥ煿鍏绘硶鍜岃娓呭鏂规硶杩涜妫€娴嬨€傞功楣夌儹琛ｅ師浣撳湪楦¤儦鍗甸粍鍥婂強HeLa缁嗚優銆佺尨鑲剧粏鑳炲煿鍏讳腑鏄撲簬鐢熼暱,浣嗙敱浜庤€楁椂璐瑰姏锛屽瀹為獙鑰呮妧鑳藉拰璁惧瑕佹眰涔熸瘮杈冮珮锛屽洜姝ゅ煿鍏绘硶寰堝皯浣跨敤銆備竴浜涜娓呭鏂规硶锛屽锛氳ˉ浣撶粨鍚堣瘯楠屻€侀棿鎺ヨ鍑濊瘯楠屾妧鏈€丒LISA銆佸井閲忓厤鐤崸鍏夋硶锛屽父鐢ㄤ簬妫€娴嬮功楣夌儹琛ｅ師浣擄紝鍏剁己鐐瑰湪浜庡彲鑳戒笌鍏朵粬琛ｅ師浣撲骇鐢熶氦鍙夊弽搴旓紝涓旂伒鏁忓害杈冧綆锛屼笉閫傜敤浜庢棭鏈熸劅鏌撱€侾CR娉曟槸涓€绉嶄綋澶栭叾淇冨悎鎴愮壒寮傛€NA 鐗囨鐨勬柟娉?鏇撮€傚悎妫€娴嬮功楣夌儹琛ｅ師浣擄紝鍏剁伒鏁忓害楂橈紝涓旂壒寮傛€у己锛屾娴嬮€熷害蹇紝鍙渶涓や笁涓皬鏃跺嵆鍙畬鎴愩€傚畾閲廝CR娉曚笌鏅€歅CR娉曠浉姣旓紝鎿嶄綔鏇翠负鏂逛究锛屾洿灏戝彈鐜姹℃煋鐨勫奖鍝嶃€傚畾閲廝CR娉曞張鍒嗕负鏌撴枡鍜屾帰閽堜袱绉嶆寚绀烘柟寮忥紝鍦ㄤ繚璇佸紩鐗╃壒寮傛€х殑鍓嶆彁涓嬶紝鏌撴枡娉曞彲浠ョ敤鏇翠綆鐨勬垚鏈揪鍒板悓鏍风殑妫€娴嬫晥鏋溿€侟/span>

鏈瘯鍓傜洅閲囩敤鏌撴枡娉曞畾閲廝CR锛岄拡瀵?6S-23S rRNA鍩哄洜鍖洪棿搴忓垪璁捐寮曠墿锛岀壒寮傛€ц瘑鍒功楣夌儹琛ｅ師浣擄紝缁廈LAST楠岃瘉锛屼笌鍏朵粬鐢熺墿鍩哄洜缁勬病鏈変氦鍙夊弽搴斻€備娇鐢ㄦ湰璇曞墏鐩掓娴嬩簡9绉嶄笉鍚岀殑琛ｅ師浣擄紝鍜屽彲鑳藉紩璧风被浼肩棁鐘剁殑20绉嶇粏鑿屽拰4绉嶇梾姣掞紝鏈彂鐜伴潪鐗瑰紓鎬т俊鍙凤紱瀵?27涓笩绫绘牱鏈繘琛屾娴嬶紝鑾峰緱47涓槼鎬с€傚彲瑙佹湰璇曞墏鐩掗€傜敤浜庨功楣夌儹琛ｅ師浣撶殑妫€娴嬪拰閴村畾銆侟/span>

鏈骇鍝佷粎渚涚爺绌朵娇鐢ㄣ€侟/span>