鎺㈤拡娉曡。鍘熶綋鐩€氱敤瀹炴椂瀹氶噺PCR璇曞墏鐩扅/span>

Probe-quantitative Real-time PCR Kit forChlamydialesorder

璐у彿锛欳qt-ord-20 瑙勬牸锛?0涓弽搴擖/span>

璐у彿锛欳qt-ord-50 瑙勬牸锛?0涓弽搴擖/span>

璐у彿锛欳qt-ord-100 瑙勬牸锛?00涓弽搴擖/span>

鍌ㄥ瓨锛?20搴︼紝閬垮厜淇濆瓨锛屾湁鏁堟湡涓€骞淬€傞伩鍏嶅弽澶嶅喕铻嶃€侟/span>

璇曞墏鐩掔壒鐐癸細

1锛?nbsp; 鐗瑰紓鎬ф娴嬭。鍘熶綋鐩井鐢熺墿锛屽寘鎷。鍘熶綋銆佸壇琛ｅ師浣撱€佽タ闂ㄥ潕姘忚弻鍜岀煶寰疯弻锛屼笌琛ｅ師浣撶洰浠ュ鐨勫叾浠栫敓鐗╁熀鍥犵粍鏃犱氦鍙夊弽搴斻€侟/span>

2锛?nbsp; 鐏垫晱搴﹂珮锛屾渶浣庢娴嬫瀬闄愪綆浜?0涓熀鍥犵粍/鍙嶅簲銆侟/span>

3锛?nbsp; DNA鑱氬悎閰堕噰鐢ㄧ儹鍚姩鏂瑰紡锛屽彲鎶戝埗闈炵壒寮傛€ф墿澧烇紝闄嶄綆鑳屾櫙鑽у厜銆侟/span>

4锛?nbsp; 甯︽湁闃虫€у鐓ф牱鍝侊紝鍙敤浜庢楠岃瘯鍓傜洅鏈夋晥鎬с€侟/span>

5锛?nbsp; 甯︽湁UDG閰跺拰dUTP锛屽彲闄嶄綆娈嬬暀DNA鐨勬薄鏌撱€侟/span>

琛ｅ師浣撶洰锛圏em>Chlamydialesorder 锛変负涓撴€х粏鑳炲唴瀵勭敓鑿岋紝鍖呮嫭7涓富瑕佺殑瀹舵棌鎴栧鏃忔€ц氨绯伙細琛ｅ師浣撶锛圏em>Chlamydiaceae锛夛紝鍓。鍘熶綋绉戯紙Parachlamydiaceae锛夛紝Criblamydiaceae锛孅em>Piscichlamydiaceae锛孅em>Rhabdochlamydiaceae, 瑗块棬鍧庢皬鑿岀锛圏em>Simkaniaceae锛夛紝鍜屽崕璇婁綋绉戯紙鎴栫О鐭冲痉鑿岀锛孅em>Waddliaceae锛夈€傞櫎浜嗚。鍘熶綋绉戯紝鍏朵粬缁嗚弻绗肩粺绉颁负绫昏。鍘熶綋銆傝。鍘熶綋绉戝井鐢熺墿鍦ㄤ汉鎴栧姩鐗╀腑閮藉睘浜庤嚧鐥呰弻銆傜被琛ｅ師浣撲篃灞炰簬涓撴€х粏鑳炲唴瀵勭敓锛岀洰鍓嶇殑鐮旂┒杈冨皯锛屽叾涓竴浜涙垚鍛樹篃涓庝汉鎴栧姩鐗╃殑鐤剧梾鏈夊叧鑱斻€侟em>Parachlamydia acanthamoebae涓庝汉绫昏偤鐐庢湁鍏筹紝杩樺彲鑳藉湪鐗涘紩璧锋祦浜э紱Simkania negevensis鍙紩璧峰懠鍚搁亾鎰熸煋锛屽挨鍏舵槸鍎跨褰撲腑锛汓em>Waddlia chondrophila寮曡捣鐗涚殑娴佷骇锛屽苟涓斿緢鍙兘鍦ㄤ汉涔熷悓鏍峰姝ゃ€備竴浜涜兘鎶垫姉闃跨背宸村師铏殑绫昏。鍘熶綋涔熷悓鏍峰浜虹殑宸ㄥ櫖缁嗚優鏈夋姉鎬с€傚洜姝わ紝妫€娴嬭繖浜涜。鍘熶綋鍜岀被琛ｅ師浣撳浜虹被鍜屽姩鐗╃柧鐥呯殑棰勯槻鍜屾不鐤楁湁閲嶈鐨勬剰涔夈€侟/span>

琛ｅ師浣撳彲浠ラ€氳繃鍩瑰吇娉曞拰琛€娓呭鏂规硶杩涜妫€娴嬨€傜敱浜庤。鍘熶綋鏄笓鎬х粏鑳炲唴瀵勭敓锛屽洜姝ゅ繀椤诲湪娲荤粍缁囨垨缁嗚優鍐呰繘琛屽煿鍏伙紝鎿嶄綔澶嶆潅锛岃€楁椂锛屼笖鐏垫晱搴︿綆銆傝娓呭鏂规硶鐨勭伒鏁忓害杈冧綆锛屼笖瀹规槗浜х敓浜ゅ弶鍙嶅簲銆傜被琛ｅ師浣撲笉鑳介噰鐢ㄤ紶缁熺殑鍩瑰吇娉曡繘琛屾娴嬨€侾CR娉曞叿鏈夌伒鏁忓害楂樸€佺壒寮傛€у己鐨勪紭鐐癸紝妫€娴嬮€熷害蹇紝鍙渶涓や笁涓皬鏃跺嵆鍙畬鎴愶紝鍙互鏈夋晥寮ヨˉ涓婅堪涓ょ鏂规硶鐨勪笉瓒炽€傚畾閲廝CR娉曚笌鏅€歅CR娉曠浉姣旓紝涓嶄粎鍙互绮剧‘瀹氶噺锛岃€屼笖鎿嶄綔鏇翠负鏂逛究锛屾洿灏戝彈鐜姹℃煋鐨勫奖鍝嶃€侟/span>

鏈瘯鍓傜洅閽堝16S rRNA鍩哄洜璁捐鐗瑰紓鎬у紩鐗╁拰鎺㈤拡锛屽彲浠ョ壒寮傛€ц瘑鍒。鍘熶綋鐩殑鍚勭寰敓鐗╋紝鍖呮嫭鍓嶈堪7绉嶅鏃忋€佽氨绯伙紝缁廈LAST楠岃瘉锛屼笌琛ｅ師浣撶洰浠ュ鐨勫叾浠栫敓鐗╁熀鍥犵粍娌℃湁浜ゅ弶鍙嶅簲銆傛湰璇曞墏鐩掓娴嬩簡6绉嶈。鍘熶綋锛?绉嶇被琛ｅ師浣擄紝9绉嶇粏鑿岋紝浠ュ強4绉嶉樋绫冲反鍘熻櫕锛屽彧鏈夎。鍘熶綋鍜岀被琛ｅ師浣撲骇鐢熺壒寮傛€т俊鍙凤紝涓庡叾浠栫粏鑿屽拰闃跨背宸村師铏病鏈変氦鍙夊弽搴斻€傚422涓蓟鍜芥嫮瀛愯繘琛屾娴嬶紝鑾峰緱48涓槼鎬э紝涓庢祴搴忕粨鏋滀竴鑷淬€傚彲瑙佹湰璇曞墏鐩掗€傜敤浜庡琛ｅ師浣撶洰寰敓鐗╃殑妫€娴嬨€侟/span>

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