瓒呮晱鍨婞/span>ECL鍖栧鍙戝厜搴曠墿璇曞墏鐩扅/span>

Super-sensitive Enhanced Chemiluminescence Substrate Kit

璇曞墏鐩掔粍鎴愶細

璐у彿	缁勪唤	鏁伴噺
ECL-P-100	A娑秉/span>	50mL
	B娑秉/span>	50mL
	璇存槑涔?/span>	1浠键/span>
ECL-P-500	A娑秉/span>	250mL
	B娑秉/span>	250mL
	璇存槑涔?/span>	1浠键/span>

鍌ㄥ瓨锛氶伩鍏変繚瀛橈紝瀹ゆ俯鍙繚鎸佺ǔ瀹氫袱骞翠互涓婏紱闀挎湡涓嶇敤锛屽缓璁疆浜嶞/span>4 oC淇濆瓨寤堕暱鏈夋晥鏈熴€侟/span>

涓€銆?strong>浜у搧绠€浠婞/strong>锛欬/span>

鏈瘯鍓傜洅鏄寲瀛﹀彂鍏夌郴缁燂紝鐢ㄤ簬妫€娴嬪浐瀹氬湪鑶滀笂鐨勮泲鐧芥垨鏍搁吀锛岀伒鏁忓害楂橈紝鑳屾櫙浣庯紝妫€娴嬬伒鏁忓害鍙揪楂橀鍏婞/span>(femtogram)绾э紝閫傜敤浜庢娴嬬棔閲忚泲鐧芥垨鏍搁吀銆傚彂鍏変俊鍙锋寔涔咃紝鎵€閲囩敤鐨勭嫭鐗归厤鏂硅冻浠ョ淮鎸?/span>6灏忔椂浠ヤ笂鐨勫彂鍏夛紝渚夸簬鍙嶅鏇濆厜鎿嶄綔銆備笌鏅€欬/span>ECL鍖栧鍙戝厜搴曠墿鐩告瘮锛屽彲鏄庢樉鍑忓皯涓€鎶楀拰浜屾姉鐨勪娇鐢ㄩ噺銆侟/span>

浜屻€佸師鐞嗭細

瓒呮晱鍨婞/span>ECL鍖栧鍙戝厜搴曠墿璇曞墏鐩掔敤浜庢娴嬬洿鎺ユ垨闂存帴鏍囪杈ｆ牴杩囨哀鍖栫墿閰讹紙HRP锛夌殑鎶椾綋銆佹姉鍘熸垨鑰呮牳閰搞€傝泲鐧借川鎴栨牳閰稿湪鐢垫吵鍚庤浆绉诲埌鍗拌抗鑶滀笂锛屼互HRP鏍囪鐨勬姉浣撴垨鎺㈤拡缁撳悎鑶滀笂鐨勭洰鐨勮泲鐧芥垨鏍搁吀锛屾礂鑶滃悗缃簬鐢ㄦ湰浜у搧閰嶅埗鐨凕/span>ECL宸ヤ綔娑蹭腑锛屽娓╁鑲叉暟鍒嗛挓锛孅/span>HRP浣垮伐浣滄恫涓殑椴佺背璇猴紙Luminol锛夋哀鍖栧苟鍙戝厜锛岃瘯鍓備腑娣诲姞鐨勫寮哄墏鍙互浣垮緱杩欑鍙戝厜澶уぇ澧炲己銆傛鍏夌粡X鍏夎兌鐗囨垨鑰呯數瀛愯缃劅鍏夎褰曚笅鏉ワ紝鍙竻鏅版樉绀鸿泲鐧借川鎴栨牳閰告潯甯︺€侟/span>

涓夈€佷骇鍝佷紭鐐癸細

楂橀鍏嬬骇鐏垫晱搴?/span>鈥斺€擖/span>楂樼伒鏁忔€э紝鑳藉揩閫熸娴嬫洿骞挎硾鐨勮泲鐧借寖鍥达紝鍙娴嬩綆杈剧害300椋炲厠鐨勮泲鐧介噺銆侟/span>

淇″彿绋冲畾鈥斺€擖/span>鍙戝厜鏃堕棿闀匡紝鑳借繘琛屽娆℃洕鍏夛紝30min鍚庝粛鍙淮鎸佺害90%鐨勫彂鍏夊己搴︼紝6灏忔椂鍚庝粛鑳界淮鎸?/span>60%浠ヤ笂鐨勫彂鍏夊己搴︺€侟/span>

淇″彿寮裹/span>鈥斺€擖/span>鍙戝厜淇″彿姣斿纰樿嫰閰氬寮虹殑HRP-椴佺背璇烘娴嬩綋绯绘洿寮恒€侟/span>

绋冲畾鎬ч珮鈥斺€擖/span>璇曞墏鐩掑彲鍦ㄥ娓╅暱鏈熺ǔ瀹氬瓨鏀俱€侟/span>

鑺傜害鎶椾綋鈥斺€擖/span>闇€瑕佹洿灏戯紙绋€閲婃洿澶氾級鐨勪竴鎶楀拰浜屾姉锛岀壒鍒妭鐪佹姉浣掽/span>(鎺ㄨ崘鐢ㄩ噺锛氫竴鎶桙/span>0.02-1.0 渭g/mL锛屼簩鎶桙/span>4-20 ng/mL)銆侟/span>

鍥涖€佷娇鐢ㄦ柟娉曪細

1.鍗拌抗鑶滃埗澶囷細鎵ц甯歌鐢垫吵銆佽浆鑶溿€?/span>HRP鏍囪鐨勬姉浣撴垨鑰匋/span>HRP鏍囪鐨勬牳閰告帰閽堝鑲层€佹礂鑶滐紱ECL宸ヤ綔娑插惈鏈堻/span>HRP鍌寲鐨勫彂鍏夊簳鐗╋紝妫€娴嬬郴缁熷繀椤诲熀浜嶞/span>HRP閰舵爣璁扮殑鎶椾綋鎴栬€呮牳閰告帰閽堛€傚厖鍒嗙殑娲楁钉瀵逛簬闄嶄綆鑳屾櫙闈炲父閲嶈锛屾墍鏈夋楠ゅ潎鍦ㄥ娓╀笅瀹屾垚銆侟/span>

2. ECL宸ヤ綔娑茬殑閰嶇疆锛氬湪浣跨敤鍓嶅彇绛夐噺A娑插拰B娑诧紝娣峰悎鍧囧寑骞跺敖蹇娇鐢紱灏嗚啘鐗囩疆娣峰悎娑蹭腑锛屼簬瀹ゆ俯涓嬪鑲茬害1鍒嗛挓锛屾瘡骞虫柟鍘樼背鑶滅墖鑷冲皯浣跨敤0.1-0.2 ml浠ヨ鐩栧叏鑶滅墖锛屾敞鎰忎笉瑕佸湪鑶滅墖琛ㄩ潰褰㈡垚姘旀场銆侟/span>

3.铔嬬櫧鎴栨牳閰镐俊鍙锋樉鐜帮細

1).鐢ㄥ钩澶撮晩閽充綇鑶滅墖锛屽瀭鐩寸疆浜庡惛姘寸焊涓婁互鍚稿幓杩囬噺璇曞墏锛屼粎鐣欎笅灏戦噺娑蹭綋瑕嗙洊琛ㄩ潰锛屼笉鍙鑶滃畬鍏ㄥ共鐕ワ紱

2).灏嗚啘鐗囩疆浜庝繚椴滆啘涓婏紝鍚搁檮铔嬬櫧闈㈡湞涓婏紝灏忓績璧跺敖姘旀场锛汓/span>

3).鐢ㄧ數瀛愯缃劅鍏夛紝鎴栧湪鏆楀涓敤X鍏夌墖鏇濆厜锛汓/span>

4).鏍规嵁淇″彿寮哄急閫傚綋璋冩暣鏇濆厜鏃堕棿锛屼篃鍙€夋嫨涓嶅悓鏃堕棿澶氭鏇濆厜锛屼互鍙栧緱鏇翠匠鏁堟灉銆侟/span>

浜斻€佸畨鍏ㄦ€э細

鏃犵壒娈婃瘨鎬э紝鎸夋櫘閫氬寲瀛﹀搧澶勭悊銆傚鏋滀笉鎱庝笌鐪笺€佺毊鑲ゅ拰琛ｇ墿鎺ヨЕ锛岃绔嬪埢鐢ㄥぇ閲忔竻姘村啿娲椼€侟/span>

鍏€佹敞鎰忎簨椤癸細

1锛庣敱浜庝笉鍚屽搧鐗岀殑鎶椾綋璐ㄩ噺涓嶅悓锛屼互鍙婃姉浣撶殑淇濆瓨鏉′欢鍜屼繚瀛樻椂闂寸殑涓嶅悓锛屽垵娆′娇鐢ㄦ椂寤鸿瀵规姉浣撶敤閲忚繘琛屼紭鍖栵紝浠ュ彇寰楁洿浼樻晥鏋溿€侟/span>

2锛庡嬁灏咟/span>ECL鍖栧鍙戝厜搴曠墿璇曞墏鐩掓毚闇插湪闃冲厜鎴栧己鍏変笅锛屽惁鍒欎細瀵艰嚧鍏跺け娲伙紱寤鸿淇濆瓨鍦ㄦ鑹茬摱涓紝骞堕伩鍏嶉暱鏃堕棿鏆撮湶鍦ㄩ槼鍏変笅锛屽疄楠屽鍏夌収瀵硅瘯鍓傜洅褰卞搷涓嶅ぇ锛涢櫎浜咟/span>X鍏夎兌鐗囨洕鍏夊拰娲楃墖澶勭悊澶栵紝鎵€鏈夋楠ゅ潎涓嶅繀鍦ㄦ殫瀹や腑鎿嶄綔銆侟/span>

3锛庝娇鐢?span style="letter-spacing:-0">鍏匋/span>瓒颤span style="letter-spacing: -0">鐨凕/span>娲桙span style="letter-spacing:-0">娑｜/span>缂掽span style="letter-spacing:-0">鍐秉/span>娑秉span style="letter-spacing:-2px">銆?/span>灏侀棴娑秉/span>銆?/span>鎶桙/span>浣掽span style="letter-spacing:-0">绋€閲夵span style="letter-spacing:-0">娑秉/span>鍜孅span style="letter-spacing:-0">搴旤/span>鐗╁伐浣淈/span>娑秉span style="letter-spacing:-0">鍘狐/span>瑕咟span style="letter-spacing:-0">鐩朁/span>鍗?span style="letter-spacing:-0">杩更/span>鑶淈span style="letter-spacing:-2px">锛孅/span>浠ョ‘淇滭/span>鍗?span style="letter-spacing:-0">杩更/span>鑶淈span style="letter-spacing:-0">澶凕/span>浜嶞span style="letter-spacing:-0">婀军/span>娑?span style="letter-spacing:-0">鐘舵€侊紱浣跨敤澶ч噺鐨勫皝闂恫鍜屾礂娑ゆ恫鑳藉噺灏戦潪鐗瑰紓鎬т俊鍙风殑浜х敓銆侟/span>

4锛庝娇鐢ㄥ墠閰嶅埗ECL宸ヤ綔娑诧紝浣撶Н瓒冲瑕嗙洊鑶滅墖鍗冲彲锛涘彇A娑插拰B娑叉椂涓€瀹氳鐢ㄤ笉鍚岀殑鏋ご锛屼簰鐩告薄鏌撳彲鑳藉鑷寸紦鎱㈠け娲伙紱閰嶅埗瀹屾瘯寤鸿绔嬪嵆浣跨敤宸ヤ綔娑诧紝瀹ゆ俯鏀剧疆鏁板皬鏃跺悗浠嶅彲浣跨敤浣嗙伒鏁忓害鐣ユ湁闄嶄綆锛涘純鍘讳娇鐢ㄨ繃鐨勬贩鍚堣瘯鍓傦紱

5锛庡嵃杩硅啘涓嶞/span>ECL宸ヤ綔娑插鑲插悗绾?/span>2 min鏃跺彂鍑虹殑鍏夋渶寮猴紝鐒跺悗闅忕潃鏃堕棿鐨勫欢闀胯€岀紦鎱㈠噺寮憋紝鑷颤/span>30 min浠嶅彲淇濇寔90%鐨勫厜淇″彿锛岃嚦24灏忔椂浠嶅彲淇濇寔绾?/span>60%鐨勫厜淇″彿锛涜泲鐧界偣鑽у厜杈冨急鏃跺彲浠ラ€傚綋寤堕暱鏇濆厜鏃堕棿锛涗娇鐢ㄨ繃灏戠殑ECL宸ヤ綔娑蹭笉鍒╁弽搴旇繘琛岋紝涓鸿揪鑺傜害鐩殑鍙皢鑶滃壀灏忥紝浣嗗嬁闄嶄綆ECL宸ヤ綔娑蹭娇鐢ㄦ瘮渚嬨€侟/span>

6锛嶞span style="letter-spacing:-0">浣跨敤鐢熺墿绱燑/span>/浜插拰绱犱綋绯绘椂锛岄伩鍏嶄娇鐢ㄨ劚鑴傚ザ绮変綔涓哄皝闂恫锛屽洜涓鸿劚鑴傚ザ绮変腑鍚湁澶氱鍐呮簮鎬х敓鐗╃礌锛屽鏄撲骇鐢熼潪鐗瑰紓鎬т俊鍙枫€侟/span>

7.閬垮厤灏嗗寮犺啘缃簬鍚屼竴涓礂鑶滅洅鍐呮礂鑶滐紝鐩镐簰鍚搁檮鎴栨懇鎿﹀彲鑳介€犳垚寰堟繁鐨勮儗鏅€侟/span>

8.閲戝睘姘у寲鐗╅绮掑彲鑳戒細閫犳垚鑶滀笂鍑虹幇棰楃矑鐘舵枒鐐癸紝閬垮厤浣跨敤甯︽湁閿堣抗鐨勫壀鍒€浠ュ強闀婂瓙锛屽缓璁娇鐢ㄥ鏂欑殑骞冲ご闀婂瓙銆侟/span>

9锛庡彔姘寲閽狅紙NaN3锛夎兘鎶戝埗HRP娲绘€э紝搴旈伩鍏嶄娇鐢?/span>NaN3锛屽蹇呴』浣跨敤锛屾祿搴︿笉瑕佽秴杩嘃/span>0.01%銆侟/span>

10.涓轰簡鎮ㄧ殑瀹夊叏鍜屽仴搴凤紝璇风┛瀹為獙鏈嶅苟鎴翠竴娆℃€ф墜濂楁搷浣滐紱鎴存墜濂楀彲浠ラ伩鍏嶅湪鑶滀笂鐣欎笅鎵嬪嵃锛屼繚鎸佽啘鐨勫共鍑€銆侟/span>

11锛庢湰浜у搧浠呬緵鐮旂┒浣跨敤銆侟/span>