涓浗鐨勫浗瀹舵爣鍑咷B2763-2016銆婇鍝佷腑鍐滆嵂鏈€澶ф畫鐣欓檺閲忋€嬩腑瑙勫畾锛屾盁铏厛鍦ㄦ鐗╂簮鎬ч鍝佷腑闄愰噺鍦?.02-0.1mg/kg涔嬮棿锛屽叾涓浮铔嬬殑闄愰噺涓?.02mg/kg锛屾棩鏈偗瀹氬垪琛ㄤ腑瑙勫畾楦¤泲涓盁铏厛鏈€澶ф畫鐣欓檺閲忎负0.02mg/kg锛屾鐩熸渶涓ユ牸锛屽湪锛圗U锛塏o.1127/2014涓瀹氳泲绫讳腑姘熻櫕鑵堢殑娈嬬暀闄愰噺涓?.005mg/kg銆侟/span>

鏍峰搧鍓嶅鐞嗘柟娉曪細

鍙?g楦¤泲鎴栭浮鑲夛紙绮剧‘鑷?.01g锛夎瘯鏍蜂簬50mL绂诲績绠′腑锛屽姞鍏?0mL涔欒厛锛屾丁鏃?0s锛岃秴澹版彁鍙?0min锛??C涓?0000 r/min绂诲績5 min锛屽皢涓婃竻娑茶浆绉昏嚦鍚湁1g NaCl鐨?5mL绂诲績绠′腑锛屾憞鍖€鍚庣蹇? min锛屽彇涓婂眰2mL閫氳繃PRiME HLB锛?cc锛 60mg锛夊皬鏌憋紝娴佸嚭娑蹭緵LC-MS/MS娴嬪畾銆侟/span>

浠櫒鍙婂弬鏁帮細

浠櫒鍙婂瀷鍙凤細宀涙触LCMS-8060娑茬浉鑹茶氨涓夐噸鍥涙瀬鏉嗚川璋包/span>

绂诲瓙婧愶細鐢靛柗闆剧瀛愭簮锛圗SI-锛夛紱绂诲瓙婧愭帴鍙ｇ數鍘嬶細1.5kV锛涢浘鍖栨皵涓烘爱姘旓紝3.0 L/min锛涘姞鐑皵涓虹┖姘旓紝10 L/min锛涘共鐕ユ皵涓烘爱姘旓紝10 L/min锛涚鎾炴皵涓烘癌姘旓紱绂诲瓙婧愭帴鍙ｆ俯搴︼細300鈩冿紱鑴辨憾鍓傜娓╁害锛?50鈩冿紱鍔犵儹妯″潡娓╁害锛?00鈩冿紱鎵弿妯″紡涓哄鍙嶅簲鐩戞祴锛圡RM锛夛紱寤惰繜鏃堕棿锛? ms锛汳RM鍙傛暟锛氳琛?銆侟/span>

鐗?/span>	姣嶇瀛?m/z)	瀛愮瀛?m/z)	纰版挒
			Q1 (V)	鑳介噺(V)	Q3 (V)
姘熻櫕鑵圏/span>	435.0	250.0	16	28	15
		330.0	21	17	10
姘熺敳鑵圏/span>	387.0	351.0	11	15	11
		282.0	14	31	12
姘熻櫕鑵堢牅	451.0	415.0	12	17	13
		282.0	17	28	17
姘熻櫕鑵堜簹鐮淈/span>	419.0	262.0	16	28	15
		383.0	21	17	10

娑茬浉鑹茶氨鏉′欢

鑹茶氨鏌憋細Waters ACQUITY UPLC?BEH C18锛?.7 渭m锛?.1 mm 脳 100 mm锛夛紱杩涙牱浣撶Н锛?渭L锛涙祦閫燂細0.3 mL/min锛涜壊璋辨煴娓╁害锛?0鈩冦€傛祦鍔ㄧ浉A锛氭按锛屾祦鍔ㄧ浉B锛氫箼鑵堬紝姊害娲楄劚B鐩稿垵濮嬫祿搴︿负60%锛堣瑙佽〃2锛夈€傚悇鐩爣鍖栧悎鐗╄壊璋卞浘瑙佸浘1-1銆侟/span>

琛? 姊害娲楄劚绋嬪簭

鏃堕棿/min	娴佸姩鐩 A/%	娴佸姩鐩 B/%
0	40	60
0.5	40	60
3	0	100
3.5	0	100
3.6	40	60
5.5	40	60

瀹為獙缁撴灉锛欬/strong>

鍥? 姘熻櫕鑵堝強鍏朵笁绉嶄唬璋骇鐗╄氨鍥撅紙5 渭g/L锛堻/span>

鍔犳爣娴撳害涓?.2-20.0 ug/kg鏃讹紝璇ユ柟娉曞楦¤泲鍜岄浮鑲夌殑鍔犳爣鍥炴敹鐜囦负81-105%涔嬮棿锛?绉嶇洰鏍囩墿鐨勫畾閲忛檺涓?.1ug/kg銆備娇鐢↙CMS-8060鍙互蹇€熷噯纭湴妫€娴嬪嚭楦¤泲涓殑姘熻櫕鑵堝強鍏朵笁绉嶄唬璋㈢墿銆侟/span>