质粒名称:tet-pLKO.puro_shGFP
质粒编号:110421
质粒备注:
shGFP control, silence GFP gene, doxycycline inducible, puromycin selection
载体名称:Tet-pLKO-puro
载体大小:10633
完整质粒大小:8816
载体类型:Mammalian Expression, Lentiviral, RNAi
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:GFP
物种来源:H. sapiens (human)
启动子:H1/TO (RNA PolIII)
克隆方法:限制性内切酶
5'克隆位点:AgeI (destroyed during cloning)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:GACAGAGACAGATCCATTCG
质粒图谱: