质粒名称:pU6-Sp-pegRNA-HEK3_CTT_ins
质粒编号:132778
质粒备注:
S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing
载体名称:pU6
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:HEK3_CTT_ins pegRNA
物种来源:Synthetic
基因突变:See manuscript
插入大小:821
启动子:U6
克隆方法:Ligation Independent Cloning
5'测序引物:GACTATCATATGCTTACCGT
质粒图谱: