质粒名称:pLBH559_Tet-HisCas14a1Locus
质粒编号:112502
质粒备注:
Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter
载体名称:pTet
载体类型:Bacterial Expression, CRISPR
原核抗性:氯霉素25μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:低拷贝
插入基因:Cas14a1
基因别名:Un1Cas12f1
插入大小:2181
启动子:Tet
克隆方法:Gibson Cloning
5'测序引物:gtgccgatcaacgtAtcattttcg
3'测序引物:acgcagaaaggcccacccgaag
质粒图谱: