质粒名称:pET42b-BE3
质粒编号:87437
质粒备注:
Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression
载体名称:pET_42b
载体大小:4983
完整质粒大小:10146
载体类型:原核表达
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:BE3
基因别名:GGS-His6-rAPOBEC1-XTEN-nCas9-GGS-UGI-GGS-NLS
物种来源:H. sapiens (human), R. norvegicus (rat); Bacillus phage PBS2
插入大小:5163
基因名称:Apobec1 (a.k.a. REPR, apobec-1)
启动子:T7
克隆方法:Ligation Independent Cloning
5'测序引物:TAATACGACTCACTATAGGG
3'测序引物:GCTAGTTATTGCTCAGCGG
质粒图谱: