质粒名称:pET42b-ABE7.10
质粒编号:120398
质粒备注:
Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression
载体名称:pET42b
载体大小:5248
完整质粒大小:10570
载体类型:原核表达
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:ABE7.10
基因别名:His6-TadA-SGGSSGGS-XTEN-SGGSSGGS-TadA*-SGGSSGGS-XTEN-SGGSSGGS-nCas9-SGGS-NLS
物种来源:Synthetic
基因突变:Mammalian codon-optimized
插入大小:5322
启动子:T7
克隆方法:限制性内切酶
5'克隆位点:Unknown (unknown if destroyed)
3'克隆位点:Unknown (unknown if destroyed)
5'测序引物:TAATACGACTCACTATAGGG
3'测序引物:GCTAGTTATTGCTCAGCGG
质粒图谱: