质粒名称:pdCas9-DNMT3A-EGFP
质粒编号:71666
质粒备注:
Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
载体名称:PX462
载体大小:4246
完整质粒大小:10266
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP
基因别名:dCas9-DNMT3A-EGFP
基因别名:SpdCas9-DNMT3A-T2A-EGFP
基因别名:dCas9-DNMT3A
物种来源:H. sapiens (human), Synthetic; S. pyogenes
基因突变:D10A and H840A in S.pyogenes Cas9
插入大小:6020
基因名称:DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
启动子:CBh
标签/融合蛋白:· 3xFLAG (N terminal on insert) · SV40 NLS (N terminal on insert) · T2A-EGFP (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:BamHI (not destroyed)
3'克隆位点:FseI (not destroyed)
5'测序引物:GS-linker For 5' GAAGAGGTACACCAGCACCAAAG 3'
3'测序引物:BGH Rev
质粒图谱: